Effects of neuraminidase 3 gene over-expression on proliferation and migration of chondrosarcoma cells

Objective To observe the neuraminidase 3 (NEU3) expression and location in SW1353 cells and investigate the effect of NEU3 expression on the proliferation and migration of human chondrosarcoma SW1353 cells. Methods The NEU3 overexpression cell line was constructed by the lipo 3000 transfection reagent. The experiment was divided into 4 groups: UN, and no treatment was given. Blank, was added with equal dose transfection reagent.pcon, was added with no-load plasmid. pNEU3, NEU3 gene overexpression plasmid was added. Immunofluorescence was used to determine the expression of NEU3 in SW1353 cells. Different groups of cells were added the lipo3000 transfection reagent and to explore these groups' mRNA expression of NEU3 by Real-time polymerase chain reaction (PCR). The impact of NEU3 over-expression on the migration of SW1353 cells was examined by scratch test. The effect of NEU3 over-expression on the proliferation of SW1353 cells was measured by EdU proliferation test and CCK-8 assay. The difference in the NEU3 expression and cell capability was tested by two independent samples t test. Results After transfection with NEU3 gene over-expressed plasmid, Real-time PCR showed that NEU3 gene mRNA was significantly up-regulated. EdU proliferation test showed that NEU3 gene over-expression could promote proliferation of SW1353 proliferation cells [positive rate for control group: (6.56±2.29)%, (11.81±6.49)%, (8.64±3.90)%; for treatment group: (40.57±14.32)% (t=11.355, P<0.05]. The scratch test showed that NEU3 gene over-expression could enhance the migration of SW1353 cells [Remian Scratch area for control group: (18 343±367), (20 803±124), (26 472±483) dpi; for treatment group: (3 320±193) dpi, t=7.265, P<0.05]. Conclusion Over-expression of NEU3 gene can enhance proliferation and migration of human chondrosarcoma SW1353 cells. Key words: Chondrosarcoma; Sialidase; Cell proliferation; Cell migration