高直链淀粉转基因4代Adira 4木薯的生长和产量性能

E. Sudarmonowati, H. Fitriani
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引用次数: 0

摘要

通过生产第一个高直链淀粉转基因Adira 4印尼木薯,进行了改善木薯淀粉质量的尝试。本研究旨在评价转基因植株的农艺性状,采用改良的方法对转基因植株的四代进行了筛选和田间试验。通过应用生长调节剂如Atonik和改进种植程序,提高了插条的存活率和生长速度。通过比较四代选定品系的相关变量来测量生长和产量表现。只有在植物生长不良、土壤和天气不好的情况下,才需要在早期种植时蘸取1mL.L-1 Atonik溶液并遮荫。在本研究中,结果表明,当用阿托尼克处理时,A55-5和A55-15的第三代和第四代的存活率可以达到100%。根据评估的14个品系,品系A55-5是最有前途的品系之一,因为它的生长、繁殖性能和直链淀粉含量几乎是野生型的两倍,而品系A55-12的产量最高。当应用适当的基因构建和方法以获得更好的生长和产量时,这一发现将有利于功能性食品行业。
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Growth and yield performance of four generations of high amylose transgenic Adira 4 cassava
Attempt to improve cassava starch quality have been conducted by producing the first high amylose transgenic Adira 4 Indonesian cassava. This atudy aimed to evaluate agronomic performances of transgenic plants, assessment of four generations in specific containment screenhouse and limited field trial were conducted with improved method. The survival and growth rate of cuttings was enhanced by applying growth regulator such as Atonik and improving planting procedures. The growth and yield performances were measured by comparing related variables across four generations of selected lines. Dipping in 1 mL.L -1 Atonik solution with shading in the early planting were only required when the plant growth was poor and not favourable soil and weather. In this study, the result showed that survival of third and fourth generations of Lines A55-5 and A55-15 could reach 100 % when it was treated with Atonik. According to 14 lines assessed, Line A55-5 was one of the promising one due to its growth, multiplication performance and almost double amylose content compared to wild type, while Line A55-12 has the highest yield. The finding will benefit functional food industries when appropriate gene construct and approaches are applied for better growth and yield results.
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16
审稿时长
10 weeks
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