Cordycepin production by the potential fungal strains Cordyceps militaris BCC 2819 and Cordyceps cicadae BCC 19788 in submerged culture during batch and Fed-batch fermentation

Cordycepin is one of the most important bioactive compounds; the low productivity and long production cycle of cordycepin are barriers to its commercialization . The optimal media for cordycepin production by Cordyceps militaris BCC 2819 and Cordyceps cicadae BCC 19788, which are potent cordycepin - producing - fungal strains, were determined through statistical experiments . Six nutrients including glucose, adenine, glycine, alanine, casein hydrolysate and vitamin solution were found to influence the cordycepin production by C . militaris BCC 2819, while the same factors were found for the cordycepin production by C . cicadae BCC 19788 except glucose that was replaced by ammonium sulfate . The highest cordycepin production of 1,176 . 69 ± 263 . 33 mg / L was obtained by C . militaris BCC 2819 using the central composite design . The highest cordycepin production of 4,259 . 63 ± 224 . 20 mg / L was obtained by C . cicadae BCC 19788 using the central composite design . Cordycepin production in 5 L fed - batch fermentation by C . militaris BCC 2819 and C . cicadae BCC 19788 using optimized medium reached maximum production levels of 3,112 . 50 and 3,587 . 10 mg / L, respectively, accounted for more than 1 . 2 - fold compared to those in batch fermentation . Furthermore, the highest levels of the bioactive compounds; exopolysaccharide, adenosine and mannitol produced by C . militaris BCC 2819 were 43 . 90 ± 2 . 51 g / L, 2,897 . 40 ± 382 . 47 mg / L and 5,981 . 10 ± 254 . 72 mg / L, respectively . The highest levels of the bioactive compounds; exopolysaccharide, adenosine and mannitol produced by C . cicadae BCC 19788 were 38 . 10 ± 2 . 84 g / L, 3,78520 ± 165 . 70 mg / L and 6,100 . 20 ± 191 . 14 mg / L, respectively . These results demonstrated the new isolates produced high amounts of bioactive compounds, especially cordycepin . Interestingly, this process can be applied for cordycepin production for future applications or scale - up studies .