用NAA和空气棕在离体培养基水中对广东茄(Solanum tuberosum L.)的增殖

F. Mukminah, Miranty Trinawaty, Teguh Prihatin
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引用次数: 0

摘要

本研究旨在为马铃薯的体外植物繁殖寻找合适的培养基配方。2015年7月至2015年10月,在Tridinanti Palembang大学农业学院组织培养实验室进行的研究。研究设计采用完全随机设计,按析因排列,九个联合处理和五个重复,即:N1A1=0.5 ppm NAA+100 ml l-1椰子水,N1A2=0.5 ppm NAA+150 ml椰子水,N1 A3=0.5 ppm NAA+200 ml l-1椰水,N2A1=1 ppm NAA+100 ml l-2椰子水,N2A3=1 ppm NAA+200 ml l-1椰子水,N3A1=1.5 ppm NAA+100 ml l-1椰水,N3A2=1.5 ppm NAA+150 ml l-1椰水,N3A3=1.5 ppm NAA+200 ml l-1椰子水。观察参数为成芽时间、高梢时间、生根时间、根数、根长和植株寿命百分比。结果表明,浓度为1ppm的NAA处理对根长7.74cm有显著影响,浓度为100 ml的椰子水对生根有显著影响(即9.97 d),浓度为200 ml的椰汁对茎高10.27cm有显著作用。在几乎所有的参数中,NAA和椰子水之间没有显著的相互作用,除了在生根的参数上。浓度为1.5ppm的NAA和200ml l-1的椰子水对生根的同等时间(即种植后7.00天)有显著的相互作用
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MULTIPLIKASI PLANLET KENTANG (Solanum tuberosum L.) SECARA IN VITRO PADA MEDIA MS DENGAN PENAMBAHAN NAA DAN AIR KELAPA
This study aimed to find appropriate media formulations for plant propagation of potato in vitro. Research conducted at the Tissue Culture Laboratory of the Faculty of Agriculture, University of Tridinanti Palembang, beginning July 2015 until October 2015. The research design was used Completely Randomized Design arranged in factorial, with nine combined treatment and five replications ie: N1A1 = 0.5 ppm NAA + 100 ml l -1 coconut water, N1A2 = 0.5 ppm NAA + 150 ml coconut water, N1A3 = 0.5 ppm NAA + 200 ml l -1 coconut water, N2A1 = 1 ppm NAA + 100 ml l -1 coconut water, N2A2 = 1 ppm NAA + 150 ml l -1 coconut water, N2A3 = 1 ppm NAA + 200 ml l -1 coconut water, N3A1 = 1.5 ppm NAA + 100 ml l -1 coconut water, N3A2 = 1.5 ppm NAA + 150 ml l -1 coconut water, and N3A3 = 1.5 ppm NAA + 200 ml l -1 coconut water. The observation parameter are the time to established buds, shoots high, the time to established roots, number of roots, root length, and the percentage of plantlets life. The results showed that treatment of NAA at a concentration of 1 ppm affected significant on the root length 7.74 cm. Coconut water at a concentration of 100 ml days after planting significantly affected the established of root ie 9.97 days after planting and 200 ml l -1 of coconut water has a significant effect on shoot height 10.27 cm. There was no significant interaction between NAA and coconut water in almost of all parameters were observed, except on the parameter of established roots. NAA at a concentration of 1.5 ppm and 200 ml l -1 of coconut water has a significant interaction for paremeter time to established roots ie 7.00 days after planting
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