Lu Liu, W. Lai, He-yang Xu, Yujie Zeng, Qiusheng Lan, Tao Zhang
{"title":"微小RNA-502-5p对癌症DLD-1细胞功能的影响","authors":"Lu Liu, W. Lai, He-yang Xu, Yujie Zeng, Qiusheng Lan, Tao Zhang","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.025","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the effect of microRNA (miRNA, miR)-502-5p on colon cancer DLD-1 cells. \n \n \nMethods \nColon cancer cell line DLD-1 was transfected by miR-502-5p mimic, control mimic, miR-502-5p inhibitor and control inhibitor respectively. The effect of miR-502-5p on the proliferation of DLD-1 cells was detected by cell counting kit-8 (CCK-8) assay. The effect of miR-502-5p on the colony forming ability of DLD-1 cells was detected by plate-cloning assay. Transwell assay was used to evaluate the effect of miR-502-5p on the migration and invasion of colon cells. \n \n \nResults \nThe expression of miR-502-5p was significantly increased (319.31±33.16 vs. 1.08±0.22) in DLD-1 cells after transfection with miR-502-5p mimic (P 0.05), while migration [(621±90) cells vs. (174±32) cells] and invasion [(531±45) cells vs. (132±34) cells] ability were significantly increased (P 0.05), but the migration [(133±50) cells vs. (543±84) cells] and invasion [(119±45) cells vs. (458±57) cells] ability were decreased significantly (P<0.05). \n \n \nConclusion \nMiR-502-5p may promote the migration and invasion of DLD-1 cells, but has no effect on the proliferation function. \n \n \nKey words: \nColon cancer; MicroRNA-502-5p; Proliferation; Migration; Invasion","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2207-2209"},"PeriodicalIF":0.0000,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The functional effects of microRNA-502-5p on colon cancer DLD-1 cells\",\"authors\":\"Lu Liu, W. Lai, He-yang Xu, Yujie Zeng, Qiusheng Lan, Tao Zhang\",\"doi\":\"10.3760/CMA.J.ISSN.1001-9030.2019.12.025\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the effect of microRNA (miRNA, miR)-502-5p on colon cancer DLD-1 cells. \\n \\n \\nMethods \\nColon cancer cell line DLD-1 was transfected by miR-502-5p mimic, control mimic, miR-502-5p inhibitor and control inhibitor respectively. The effect of miR-502-5p on the proliferation of DLD-1 cells was detected by cell counting kit-8 (CCK-8) assay. The effect of miR-502-5p on the colony forming ability of DLD-1 cells was detected by plate-cloning assay. Transwell assay was used to evaluate the effect of miR-502-5p on the migration and invasion of colon cells. \\n \\n \\nResults \\nThe expression of miR-502-5p was significantly increased (319.31±33.16 vs. 1.08±0.22) in DLD-1 cells after transfection with miR-502-5p mimic (P 0.05), while migration [(621±90) cells vs. (174±32) cells] and invasion [(531±45) cells vs. (132±34) cells] ability were significantly increased (P 0.05), but the migration [(133±50) cells vs. (543±84) cells] and invasion [(119±45) cells vs. (458±57) cells] ability were decreased significantly (P<0.05). \\n \\n \\nConclusion \\nMiR-502-5p may promote the migration and invasion of DLD-1 cells, but has no effect on the proliferation function. \\n \\n \\nKey words: \\nColon cancer; MicroRNA-502-5p; Proliferation; Migration; Invasion\",\"PeriodicalId\":10065,\"journal\":{\"name\":\"中华实验外科杂志\",\"volume\":\"36 1\",\"pages\":\"2207-2209\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-12-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华实验外科杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.025\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验外科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.025","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The functional effects of microRNA-502-5p on colon cancer DLD-1 cells
Objective
To investigate the effect of microRNA (miRNA, miR)-502-5p on colon cancer DLD-1 cells.
Methods
Colon cancer cell line DLD-1 was transfected by miR-502-5p mimic, control mimic, miR-502-5p inhibitor and control inhibitor respectively. The effect of miR-502-5p on the proliferation of DLD-1 cells was detected by cell counting kit-8 (CCK-8) assay. The effect of miR-502-5p on the colony forming ability of DLD-1 cells was detected by plate-cloning assay. Transwell assay was used to evaluate the effect of miR-502-5p on the migration and invasion of colon cells.
Results
The expression of miR-502-5p was significantly increased (319.31±33.16 vs. 1.08±0.22) in DLD-1 cells after transfection with miR-502-5p mimic (P 0.05), while migration [(621±90) cells vs. (174±32) cells] and invasion [(531±45) cells vs. (132±34) cells] ability were significantly increased (P 0.05), but the migration [(133±50) cells vs. (543±84) cells] and invasion [(119±45) cells vs. (458±57) cells] ability were decreased significantly (P<0.05).
Conclusion
MiR-502-5p may promote the migration and invasion of DLD-1 cells, but has no effect on the proliferation function.
Key words:
Colon cancer; MicroRNA-502-5p; Proliferation; Migration; Invasion
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