无腺体棉籽与有腺体棉籽贮藏蛋白分布的定量比较

IF 2.3 4区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Agricultural & Environmental Letters Pub Date : 2022-05-11 DOI:10.1002/ael2.20076
Zhongqi He, Dunhua Zhang, Christopher P. Mattison
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引用次数: 11

摘要

带腺体的棉子(Gossypium hirsutum L.)含有分散的棉酚腺体。无腺体(Gl)棉籽是一种仅含微量棉酚的新型棉籽。本工作定量比较了Gd和Gl分离蛋白的含量和迁移模式。对两种蛋白质样品进行十二烷基硫酸钠(SDS)凝胶电泳,并将蛋白质凝胶带分为七个部分进行肽质谱分析。虽然在两个样品中都存在vicilin和legumin蛋白的多个肽片段(异构体),但vicilin在总种子蛋白中的百分比在Gd中(74.9%)高于在Gl中(63.4%)。相反,legumin蛋白在Gl(30.4%)中比Gd(23.6%)更丰富。与Gd相比,在Gl中也观察到次要蛋白质成分,如脂质相关的油蛋白和vicilin样抗菌肽2‐2的发生率相对较高,这可能反映了在缺乏棉酚的情况下,需要提高蛋白质相关防御能力,以对抗天敌或不利的生长环境。
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Quantitative comparison of the storage protein distribution in glandless and glanded cottonseeds

Glanded (Gd) cottonseed (Gossypium hirsutum L.) contains scattered gossypol glands. Glandless (Gl) cottonseed is a new type of seed containing only trace levels of gossypol. This work quantitatively compared the content and migration pattern of Gd and Gl protein isolates. Both protein samples were subjected to sodium dodecyl sulfate (SDS)-gel electrophoresis, and the protein gel bands were separated into seven partitions for peptide mass spectroscopic analysis. While multiple peptide fragments (isoformers) of vicilin and legumin proteins were present in both samples, the percentage of vicilins in total seed protein was higher in Gd (74.9%) than in Gl (63.4%). In contrast, legumin proteins were more abundant in Gl (30.4%) than Gd (23.6%). Minor protein components such as lipid-related oleosins and vicilin-like antimicrobial peptides 2-2 were also observed at a relatively higher incidence in Gl compared with Gd, potentially reflecting a need for increased protein-related defense capability in the absence of gossypol against natural predators or adverse growth environment.

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来源期刊
CiteScore
3.70
自引率
3.80%
发文量
28
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