人类记忆样NK细胞的鉴定

Q1 Health Professions Current Protocols in Cytometry Pub Date : 2017-01-05 DOI:10.1002/cpcy.13
Elena I. Kovalenko, Maria A. Streltsova, Leonid M. Kanevskiy, Sophia A. Erokhina, William G. Telford
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引用次数: 18

摘要

我们对NK生物学的理解急剧增加,这是用于分析这些细胞的改进的流式细胞术技术的产物。NK细胞在分化过程中发生了显著的库变化。重复刺激,如巨细胞病毒感染,可能导致某些类型的高度分化NK细胞的积累,称为记忆样NK细胞或适应性NK细胞。适应性NK细胞对回忆刺激具有快速扩增和有效反应的能力。这些细胞在功能和表型上都与传统NK细胞有很大的不同。在这里,我们描述了一种方法来鉴定和分析适应性NK细胞在人外周血。cd57阳性细胞具有活化受体NKG2C高表达、KIR受体表达增加、缺乏与抑制受体NKG2A共表达、活化受体NCR3 (NKp30)表达降低等特征。下面描述的流式细胞术方法可以在一个相对简单的流式细胞仪上识别NK细胞亚群。©2017 by John Wiley &儿子,Inc。
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Identification of Human Memory-Like NK Cells

Our understanding of NK biology is increased dramatically, a product of improved flow-cytometric techniques for analyzing these cells. NK cells undergo significant changes in repertoire during differentiation. A repeating stimulus, such as a cytomegalovirus infection, may result in accumulation of certain types of highly differentiated NK cells designated as memory-like, or adaptive NK cells. Adaptive NK cells are capable of rapid expansion and effective response to the recall stimulus. These cells differ significantly from conventional NK cells both functionally and phenotypically. Here we describe an approach for identification and analysis of adaptive NK cells in human peripheral blood. CD57-positive cells with high expression of activating-receptor NKG2C, increased expression of KIR receptors, lack of co-expression with inhibitory receptor NKG2A, and decreased expression of activating receptor NCR3 (NKp30) all characterize this cell type. The flow-cytometric method described below can identify this NK cell subset on a relatively simple flow cytometer. © 2017 by John Wiley & Sons, Inc.

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来源期刊
Current Protocols in Cytometry
Current Protocols in Cytometry Health Professions-Medical Laboratory Technology
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期刊介绍: Published in affiliation with the International Society for Advancement of Cytometry, Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available.
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Issue Information Detection and Sorting of Extracellular Vesicles and Viruses Using nanoFACS Live Imaging of the Lung Small Particle Fluorescence and Light Scatter Calibration Using FCMPASS Software. Issue Information
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