{"title":"由Paenibacillus幼虫诱导的新兴蜂后Apis mellifera L.抗菌基因的评估","authors":"Sas Gomaa, Ems Barakat, M. Salama, EE El Gohary","doi":"10.17159/2254-8854/2022/a13586","DOIUrl":null,"url":null,"abstract":"This study evaluated the expression of antimicrobial abaecin and defensin genes in virgin honey-bee queens (Apis mellifera) following induction of 4th larval instar with the bacterium Paenibacillus larvae larvae (1.07 × 102 CFU/queen); to investigate whether the presence of bacteria affects the immune response as well as gene transcript levels for the immune proteins. The total body proteins of bacteria-treated queens showed a highly significant increase, and the appearance of new proteins patterns and/or disappearance of others. The gene expression profile of treated queens showed up-regulation equalled four-fold of the defensin gene, whereas no progression occurred in the abacien gene. This finding likewise greatly affects the diseases challenging this pollinator.","PeriodicalId":7566,"journal":{"name":"African Entomology","volume":" ","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Assessment of antibacterial genes of emerging queen Apis mellifera L. induced by the bacterium Paenibacillus larvae larvae\",\"authors\":\"Sas Gomaa, Ems Barakat, M. Salama, EE El Gohary\",\"doi\":\"10.17159/2254-8854/2022/a13586\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study evaluated the expression of antimicrobial abaecin and defensin genes in virgin honey-bee queens (Apis mellifera) following induction of 4th larval instar with the bacterium Paenibacillus larvae larvae (1.07 × 102 CFU/queen); to investigate whether the presence of bacteria affects the immune response as well as gene transcript levels for the immune proteins. The total body proteins of bacteria-treated queens showed a highly significant increase, and the appearance of new proteins patterns and/or disappearance of others. The gene expression profile of treated queens showed up-regulation equalled four-fold of the defensin gene, whereas no progression occurred in the abacien gene. This finding likewise greatly affects the diseases challenging this pollinator.\",\"PeriodicalId\":7566,\"journal\":{\"name\":\"African Entomology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2022-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"African Entomology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.17159/2254-8854/2022/a13586\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"African Entomology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.17159/2254-8854/2022/a13586","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Assessment of antibacterial genes of emerging queen Apis mellifera L. induced by the bacterium Paenibacillus larvae larvae
This study evaluated the expression of antimicrobial abaecin and defensin genes in virgin honey-bee queens (Apis mellifera) following induction of 4th larval instar with the bacterium Paenibacillus larvae larvae (1.07 × 102 CFU/queen); to investigate whether the presence of bacteria affects the immune response as well as gene transcript levels for the immune proteins. The total body proteins of bacteria-treated queens showed a highly significant increase, and the appearance of new proteins patterns and/or disappearance of others. The gene expression profile of treated queens showed up-regulation equalled four-fold of the defensin gene, whereas no progression occurred in the abacien gene. This finding likewise greatly affects the diseases challenging this pollinator.
期刊介绍:
African Entomology (ISSN 1021-3589 – print / 2224-8854 – online) replaced the old Journal of the Entomological Society of Southern Africa in 1993. A single volume consisting of two issues (March and September) is published annually. The journal is indexed in all major abstracting journals
African Entomology is a peer reviewed scientific journal that publishes original research articles and short communications on all aspects of entomology, with an emphasis on the advancement of entomology on the African continent.