在生物传感器表面功能化框架内测定SiO2膜上的Au膜硫化和硅烷键合——最佳实践和技术分析

IF 0.7 4区 化学 Q4 CHEMISTRY, MULTIDISCIPLINARY Croatica Chemica Acta Pub Date : 2020-04-24 DOI:10.5562/cca3640
L. Bousiakou, Stefanos Karapetis
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引用次数: 1

摘要

本文综述了基于自组装单层的生物传感中一些最常见的表面修饰方法,特别关注Au膜的硫化和SiO2膜的硅烷化。这种方法通常用于改变材料的表面性质以达到所需的生物响应。此外,还介绍了确保表面改性成功的最合适的表征方法,包括XPS、HREELS、SPFS、拉曼光谱和FTIR光谱以及UPS,并举例说明了它们的重要性。此外,考虑到硫化Au膜和硅烷化SiO2在将酶、抗体或趋化因子等生物分子偶联到表面上的重要性,讨论了其荧光和非荧光生物素化的机制,这对生物传感应用具有重要意义。最后,在此框架内,讨论了确保有效附着的表征路线。
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Determination of Au Film Thiolation and Silane Bonding Onto SiO2 Films Within the Frame of Biosensor Surface Functionalization – an Analysis of Best Practices and Techniques
This paper reviews some of the most common surface modification approaches in biosensing based on self-assembled monolayers with a particular focus on Au film thiolation and SiO2 film silanization. Such approaches are routinely used to alter the materials’ surface properties towards a desired bioresponse. Furthermore, the most appropriate characterization methods towards ensuring successful surface modification are presented including XPS, HREELS, SPFS, Raman and FTIR spectroscopy as well as UPS with specific examples to demonstrate their importance. In addition, the mechanisms of fluorescent and non-fluorescent biotinylation of thiolated Au films and silanized SiO2 are discussed considering its importance in conjugating biomolecules such as enzymes, antibodies or chemokines onto surfaces, which carries high significance for biosensing applications. Finally, within this frame characterization routes towards ensuring effective attachment are discussed.
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来源期刊
Croatica Chemica Acta
Croatica Chemica Acta 化学-化学综合
CiteScore
0.60
自引率
0.00%
发文量
3
审稿时长
18 months
期刊介绍: Croatica Chemica Acta (Croat. Chem. Acta, CCA), is an international journal of the Croatian Chemical Society publishing scientific articles of general interest to chemistry.
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