从鳞翅目鳞片状卵块中分散卵子并观察胚胎发生的简单方法

IF 0.7 4区 农林科学 Q4 ENTOMOLOGY Entomological Science Pub Date : 2022-01-11 DOI:10.1111/ens.12497
Hiroshi Arai, Yuna Ishitsubo, Madoka Nakai, Maki N. Inoue
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引用次数: 2

摘要

各种昆虫在一个相连的“卵团”中产下微小、薄而柔软的卵。特别是在几种鳞翅目物种中,这种集群卵子的结构覆盖着复杂的鳞片状分泌物,迄今为止,这阻碍了对单个胚胎的分析。然而,很少有人研究分散这种昆虫卵簇的方法以及比较不同方法。为了克服这些问题,我们开发了将卵团分离成单个卵的方法,使用了两种Tortricidae害虫,Homona marita Diakonoff和Adoxophyes honmai Yasuda(鳞翅目:Tortricida)。使用氢氧化钾和次氯酸钠成功地将鸡蛋彼此分离。尽管分离的卵子不再继续其胚胎发生,但庚烷-多聚甲醛固定、庚烷-甲醇透化和几种染料染色使胚胎发生的观察变得容易。该方案预计将适用于其他昆虫分类群,并将促进对产卵群体昆虫的进一步形态学和遗传学研究。
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A simple method to disperse eggs from lepidopteran scalelike egg masses and to observe embryogenesis

Various insect species lay tiny, thin- and soft-shelled eggs in a connected “egg mass”. Especially in several lepidopteran species, the structure of such clustered eggs is covered with complicated scale-like secretion, which has so far prevented analysis of individual embryos. However, few studies on methods to disperse egg clusters of such insects and to compare different methods have been carried out. To overcome these problems, we developed methods to separate egg masses into individual eggs, using two Tortricidae pests, Homona magnanima Diakonoff and Adoxophyes honmai Yasuda (Lepidoptera: Tortricidae). The eggs were successfully separated from each other using potassium hydroxide and sodium hypochlorite. Although the separated eggs no longer continued their embryogenesis, fixation with heptane–paraformaldehyde, permeabilization with heptane–methanol, and staining with several dyes enabled easy observation of embryogenesis. This protocol is expected to be applicable to other insect taxa and will facilitate further morphological and genetic studies in insects that lay egg masses.

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来源期刊
Entomological Science
Entomological Science 生物-昆虫学
CiteScore
2.00
自引率
11.10%
发文量
30
审稿时长
6-12 weeks
期刊介绍: Entomological Science is the official English language journal of the Entomological Society of Japan. The Journal publishes original research papers and reviews from any entomological discipline or from directly allied field in ecology, behavioral biology, physiology, biochemistry, development, genetics, systematics, morphology, evolution and general entomology. Papers of applied entomology will be considered for publication if they significantly advance in the field of entomological science in the opinion of the Editors and Editorial Board.
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