STAT5介导的miR-33-5p在鸡毒支原体感染的DF-1细胞中的转录。

IF 2.5 2区 农林科学 Q1 VETERINARY SCIENCES Avian Pathology Pub Date : 2024-02-01 Epub Date: 2023-12-19 DOI:10.1080/03079457.2023.2272617
Yingfei Sun, Yingjie Wang, Lulu Wang, Mengyun Zou, Xiuli Peng
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引用次数: 0

摘要

鸡败血症支原体(MG)是鸡慢性呼吸道疾病(CRD)的主要病原体。CRD是全球家禽业的普遍疾病,造成了巨大的经济损失。在我们之前的工作中,我们报道了miR-33-5p通过特异性靶向鸡的JNK1来抑制MG诱导的细胞凋亡、炎症因子的产生和MG的复制。然而,在MG感染期间控制miR-33-5p表达的调控机制仍知之甚少。因此,本研究的目的是探索miR-33-5p基因在MG感染过程中的表达调控。在这项研究中,我们观察到MG感染后pri-miR33-5p和pre-miR-33-5p的表达上调,表明它们的调节发生在转录水平。此外,我们对miR-33-5p的转录调控区进行了表征,并发现了STAT5的结合基序的存在,STAT5是一种公认的负责基因表达调控的转录因子。萤光素酶报告基因分析和突变分析明确表明,STAT5与miR-33-5p启动子元件结合,从而调节miR-33-5p对MG感染的转录。最终,STAT5的过度表达导致miR-33-5p表达升高,同时抑制JNK1的表达。我们的研究结果阐明了STAT5在MG感染期间控制miR-33-5p的上调。本研究为全面了解MG的发病机制和探索MG诱导的CRD的治疗方法提供了依据。
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STAT5-mediated transcription of miR-33-5p in Mycoplasma gallisepticum-infected DF-1 cells.

Research highlights: MG-HS regulates the expression of transcription factor STAT5.Transcription factor STAT5 can target miR-33-5p promoter element.MG-influenced STAT5 regulates miR-33-5p and its target gene expression.

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来源期刊
Avian Pathology
Avian Pathology 农林科学-兽医学
CiteScore
4.50
自引率
10.70%
发文量
68
审稿时长
1 months
期刊介绍: Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.
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