大鼠精索静脉曲张细胞分裂周期和核糖体途径的综合生物信息学分析:药物发现的意义。

IF 1.7 4区 生物学 Q4 CELL BIOLOGY Cell Journal Pub Date : 2023-10-09 DOI:10.22074/cellj.2023.2004771.1329
Ali Nasr-Esfahani, Ali Valipour Motlagh, Minoo Adib, Kosar Pashaei, Mohammad Hossein Nasr-Esfahani
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引用次数: 0

摘要

目的:精索静脉曲张是男性不育的常见原因,影响了相当一部分不育男性。最近的研究采用转录组学分析来确定可能与这种疾病的发病机制有关的候选基因。因此,本研究试图利用大鼠基因表达谱以及蛋白质-蛋白质相互作用网络来确定关键的调控基因、相关途径和治疗精索静脉曲张的潜在有效药物。材料和方法:在这项计算机研究中,使用R软件中的edgeR软件包从3只大鼠的睾丸组织中筛选差异表达基因(DEGs),并将结果与对照组的3只大白鼠进行比较。数据来自GSE139447。设定a-11和PR结果:在本研究的1277个DEG中,与对照组相比,精索静脉曲张大鼠的677个基因上调,600个基因下调。利用蛋白质-蛋白质相互作用网络,我们确定了前五个上调基因和前五个下调基因。富集分析表明,上调的基因与细胞分裂周期途径有关,而下调的基因与核糖体途径有关。值得注意的是,我们的研究结果表明,地塞米松可能是治疗精索静脉曲张的一种有前景的选择。结论:目前的研究表明,与对照组相比,精索静脉曲张的细胞分裂周期途径上调,而核糖体途径下调。基于这些发现,地塞米松可以被认为是治疗精索静脉曲张的未来候选药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Integrative Bioinformatics Analysis of The Cell Division Cycle and Ribosomal Pathways in The Rat Varicocele: Implications for Drug Discovery.

Objective: Varicocele is a common cause of male infertility, affecting a substantial proportion of infertile men. Recent studies have employed transcriptomic analysis to identify candidate genes that may be implicated in the pathogenesis of this condition. Accordingly, this study sought to leverage rat gene expression profiling, along with protein-protein interaction networks, to identify key regulatory genes, related pathways, and potentially effective drugs for the treatment of varicocele.

Materials and methods: In this in-silico study, differentially expressed genes (DEGs) from the testicular tissue of 3 rats were screened using the edgeR package in R software and the results were compared to 3 rats in the control group. Data was obtained from GSE139447. Setting a -11 and P<0.05 as cutoff points for statistical significance, up and down-regulated genes were identified. Based on Cytoscape plugins, protein-protein interaction (PPI) networks were drawn, and hub genes were highlighted. ShinyGO was used for pathway enrichment. Finally, effective drugs were identified from the drug database.

Results: Among the 1277 DEGs in this study, 677 genes were up-regulated while 600 genes were down-regulated in rats with varicocele compared to the control group. Using protein-protein interaction networks, we identified the top five up-regulated genes and the top five down-regulated genes. Enrichment analysis showed that the up-regulated genes were associated with the cell division cycle pathway, while the down-regulated genes were linked to the ribosome pathway. Notably, our findings suggested that dexamethasone may be a promising therapeutic option for individuals with varicocele.

Conclusion: The current investigation indicates that in varicocele the cell division cycle pathway is up-regulated while the ribosome pathway is down-regulated compared to controls. Based on these findings, dexamethasone could be considered a future candidate drug for the treatment of individuals with varicocele.

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来源期刊
Cell Journal
Cell Journal CELL BIOLOGY-
CiteScore
3.40
自引率
5.00%
发文量
0
审稿时长
12 months
期刊介绍: The “Cell Journal (Yakhteh)“, formerly published as “Yakhteh Medical Journal”, is a quarterly English publication of Royan Institute. This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.
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