绿泥蟹Scylla serrata肠道微生物群细菌非核糖体和聚酮类生物合成基因的检测及其拮抗活性

Shabreen Banu , Shivakiran Alva , Prathiksha J. Prabhu , Sreedharan Krishnan , Madhu K. Mani
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引用次数: 0

摘要

被称为非核糖体肽合成酶(NRPs)和聚酮合成酶(PKs)的多模块酶复合物已在细菌中被广泛报道,这些细菌分别产生次级生物活性代谢产物,如非核糖体肽(NRPs)和聚酮类(PKs)。这些NRPS/PKS途径有助于合成几种抗生素,如万古霉素、利福霉素和博来霉素,这些抗生素在人类医学中至关重要。本研究旨在从锯缘青蟹中分离肠道相关细菌,并检测与之相关的NRPS和PKS基因簇。生物合成基因簇(NRPS和PKS)通过使用这些基因特异性的变性引物的PCR检测。三个分离株(FKP2-4、FKP4-1和FKP2-16)对NRPS呈阳性,两个对PKS(FKP2/4和FKP4-1)基因呈阳性。对分离物进行16S rRNA基因扩增并测序。在与国家生物技术信息中心(NCBI)数据库中现有的序列进行比较后,使用基本局部比对搜索工具(BLAST)对序列进行的计算机分析将分离株FKP2–4、FKP4–1和FKP2–16分别鉴定为可变不动杆菌、流淋球菌和arlettae葡萄球菌。与对照相比,观察到这些分离物对大肠杆菌和金黄色葡萄球菌表现出有趣的拮抗活性。然而,这些分离株未能显示出对白色念珠菌的显著活性。使用含有5%蔗糖的Zobell海洋琼脂(ZMA)测试分离的生物体产生的外多糖,但没有一个菌落是粘液或黏糊糊的。
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Detection of non-ribosomal and polyketide biosynthetic genes in bacteria from green mud crab Scylla serrata gut microbiome and their antagonistic activities
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