Graphene oxide decreases the effects of salt stress on Persian clover seed germination.

Among biotic and abiotic stresses, the most restrictive for plant distribution is salt stress, where different concentrations might exert harmful effects on seed germination. Recently, nanomaterials were used successfully to mitigate these stresses, indicating that plants may be able to develop normally in adverse conditions. The aim of this study was to examine the effects of graphene oxide (GO) on the germination of Persian clover seedlings under salt stress conditions. Following sown on substrate paper, seeds were tested after exposure to different concentrations of graphene oxide (0, 125, 250, or 500 mg L^-1 GO), sodium chloride (0; -0.1; -0.2; -0.3, or -0.4 MPa NaCl) and/or GO + salt concomitantly, and then stored for 7 days in a germination chamber at 20°C in the presence of light. Seed germination and growth parameters of seedlings were determined. Graphene oxide demonstrated protective effect against salt stress as evident by no marked adverse effects on seed germination where GO blocked the salt-induced reduction in germination. The results obtained provide references for the safe application of nanomaterials and emphasize the significance of GO as a promising material for reducing the toxicity of salts in agriculture.