柠檬酸钠对尼罗罗非鱼(Oreochromis niloticus)的双重作用:促进脂质和蛋白质沉积与引起高血糖和胰岛素抵抗

IF 6.3 Animal Nutrition Pub Date : 2023-09-01 DOI:10.1016/j.aninu.2023.06.005
Jun-Xian Wang, Fang Qiao, Mei-Ling Zhang, Li-Qiao Chen, Zhen-Yu Du, Yuan Luo
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引用次数: 0

摘要

柠檬酸盐是能量代谢的重要底物,在调节葡萄糖和脂质代谢稳态方面发挥着关键作用。然而,柠檬酸盐在调节鱼类营养代谢方面的作用仍知之甚少。在此,我们研究了日粮柠檬酸钠对尼罗罗非鱼幼鱼生长性能和系统能量代谢的影响。将270只尼罗罗非鱼(2.81±0.01g)随机分为三组(每组3个重复,每个重复30条鱼),分别用对照日粮(35%蛋白质和6%脂质)、2%和4%柠檬酸钠日粮喂养8周。结果表明,柠檬酸钠对生长性能无影响(P>0.05)。4%柠檬酸钠组的全身粗蛋白、血清甘油三酯和肝糖原含量显著增加(P<0.05),而2%柠檬酸钠组没有(P>0.05)。4%柠檬酸钠治疗显著提高了喂养试验结束时和糖耐量试验中的血糖和胰岛素水平(P<0.05)磷酸丙酮酸脱氢酶E1组分亚单位α蛋白(P<;0.05)。此外,4%柠檬酸钠显著增加了肝甘油三酯和乙酰辅酶A水平,而肉碱棕榈酰转移酶1a蛋白的表达被4%柠檬酸盐钠显著下调(P<)。除此之外,4%柠檬酸钠通过激活mTOR信号传导和抑制AMPK信号传导诱导肌肉中粗蛋白沉积(P<;0.05)。此外,4%柠檬酸盐显著抑制血清天冬氨酸氨基转移酶和丙氨酸氨基转移酶活性,同时降低促炎基因如nfκb的表达,tnfα和il8的表达(P<;0.05)。尽管4%柠檬酸钠显著增加了磷核因子kB p65的蛋白表达(P>;0.05),但没有发生显著的组织损伤或炎症。总之,饮食中补充柠檬酸钠可能对尼罗罗非鱼表现出双重作用,积极方面促进营养沉积,消极方面导致高血糖和胰岛素抵抗。
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Double-edged effect of sodium citrate in Nile tilapia (Oreochromis niloticus): Promoting lipid and protein deposition vs. causing hyperglycemia and insulin resistance

Citrate is an essential substrate for energy metabolism that plays critical roles in regulating glucose and lipid metabolic homeostasis. However, the action of citrate in regulating nutrient metabolism in fish remains poorly understood. Here, we investigated the effects of dietary sodium citrate on growth performance and systematic energy metabolism in juvenile Nile tilapia (Oreochromis niloticus). A total of 270 Nile tilapia (2.81 ± 0.01 g) were randomly divided into three groups (3 replicates per group, 30 fish per replicate) and fed with control diet (35% protein and 6% lipid), 2% and 4% sodium citrate diets, respectively, for 8 weeks. The results showed that sodium citrate exhibited no effect on growth performance (P > 0.05). The whole-body crude protein, serum triglyceride and hepatic glycogen contents were significantly increased in the 4% sodium citrate group (P < 0.05), but not in the 2% sodium citrate group (P > 0.05). The 4% sodium citrate treatment significantly increased the serum glucose and insulin levels at the end of feeding trial and also in the glucose tolerance test (P < 0.05). The 4% sodium citrate significantly enhanced the hepatic phosphofructokinase activity and inhibited the expression of pyruvate dehydrogenase kinase isozyme 2 and phosphor-pyruvate dehydrogenase E1 component subunit alpha proteins (P < 0.05). Additionally, the 4% sodium citrate significantly increased hepatic triglyceride and acetyl-CoA levels, while the expressions of carnitine palmitoyl transferase 1a protein were significantly down-regulated by the 4% sodium citrate (P < 0.05). Besides, the 4% sodium citrate induced crude protein deposition in muscle by activating mTOR signaling and inhibiting AMPK signaling (P < 0.05). Furthermore, the 4% sodium citrate significantly suppressed serum aspartate aminotransferase and alanine aminotransferase activities, along with the lowered expression of pro-inflammatory genes, such as nfκb, tnfα and il8 (P < 0.05). Although the 4% sodium citrate significantly increased phosphor-nuclear factor-kB p65 protein expression (P < 0.05), no significant tissue damage or inflammation occurred. Taken together, dietary supplementation of sodium citrate could exhibit a double-edged effect in Nile tilapia, with the positive aspect in promoting nutrient deposition and the negative aspect in causing hyperglycemia and insulin resistance.

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来源期刊
Animal Nutrition
Animal Nutrition Animal Science and Zoology
CiteScore
9.70
自引率
0.00%
发文量
542
审稿时长
65 days
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
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