大麦籽粒组织代谢产物的质谱成像研究

Q1 Agricultural and Biological Sciences Current protocols in plant biology Pub Date : 2016-12-01 DOI:10.1002/cppb.20037

Manuela Peukert, Wai Li Lim, Udo Seiffert, Andrea Matros

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引用次数: 13

摘要

高等植物是由许多具有特定功能的组织组成的，这反映在转录物、蛋白质和代谢物的不同特征上。尽管近年来“组学”技术的快速发展使植物科学取得了巨大的进步，但分析往往是对整个器官或整个植物提取物进行分析，造成了空间信息的丢失。基于质谱的成像(MSI)方法已成为破译空间分辨分子信息的有力工具。基质辅助激光解吸/电离(MALDI)是MSI中应用最广泛的电离方法，近年来在植物科学中得到了应用。MSI已经成功地分析了一系列不同的植物器官和组织，并获得了初级和次级代谢的各种代谢物的模式。本协议描述了一种分析空间代谢物分布的方法在冷冻发育的大麦谷物。提供了样品制备，质谱测量和数据分析的详细程序。©2016 by John Wiley &儿子,Inc。

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Mass Spectrometry Imaging of Metabolites in Barley Grain Tissues

Higher plants are composed of a multitude of tissues with particular functions, reflected by distinct profiles of transcripts, proteins, and metabolites. Although the rapid development of “omics” technologies has advanced plant science tremendously within recent years, analysis is frequently performed on whole organ or whole plant extracts, causing the loss of spatial information. Mass spectrometry–based imaging (MSI) approaches have become a powerful tool to decipher spatially resolved molecular information. Matrix-assisted laser desorption/ionization (MALDI) is the most widespread ionization method utilized for MSI and has recently been applied to plant science. A range of different plant organs and tissues has been successfully analyzed by MSI, and patterns of various classes of metabolites from primary and secondary metabolism have been obtained. This protocol describes a method for analysis of spatial metabolite distributions in cryosections of developing barley grains. Detailed procedures for sample preparation, mass spectrometry measurement, and data analysis are provided. © 2016 by John Wiley & Sons, Inc.

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Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.