虹鳟肌肉生长抑制素基因(MSTN-1)多态性与生长性状的关系

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2016-08-01 DOI:10.1016/j.aggene.2016.08.003
Sajad Nazari , Valiolah Jafari , Mohammad Pourkazemi , Hamed Kolangi Miandare , Hossein Ali Abdolhay
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引用次数: 17

摘要

本研究分析了虹鳟鱼(Oncorhynchus mykiss)数量性状候选基因肌生长抑制素基因(MSTN-1)多态性。研究了MSTN-1多态性与生长性状的关系。选取了Yasuj和Shahrkord两个养殖场的虹鳟鱼亲本40条,并进行了标记。这些产卵被交配,然后幼鱼在池塘里饲养,直到孵化后5个月。两个单核苷酸多态性(snp: g.660T >C和g.1904C >A)采用DNA测序和pcr -单链构象多态性(PCR-SSCP)方法测定虹鳟鱼MSTN-1基因内含子1和2区域的表达。评估结果表明,这两个位点突变是同义突变,因此在这些样本中发现了6个单倍型(n = 120)。此外,SNP与生长性状的单因素方差分析揭示了SNP g.1904C >A与90日龄和150日龄的体长和体重有关(P <0.05),而SNP g.660T与gt;C和生长性状参数。这些结果提供了MSTN-1基因snp可能与生命早期(从孵化到5个月)的长度和体重相关的证据,因此MSTN-1基因可能用于虹鳟育种计划。
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Association between myostatin gene (MSTN-1) polymorphism and growth traits in domesticated rainbow trout (Oncorhynchus mykiss)

In this study, the polymorphism of myostatin gene (MSTN-1) as a candidate gene for quantitative traits was analyzed in the rainbow trout (Oncorhynchus mykiss). The association between the MSTN-1 polymorphisms and growth traits were also studied. Forty broodstock parents of rainbow trout from two farms including Yasuj and Shahrkord were selected and tagged. These spawning were mated and then juveniles were raised at the ponds until 5 months post-hatching. Two single nucleotide polymorphisms (SNPs: g.660T > C and g.1904C > A) in intron 1 and 2 regions of the MSTN-1 gene in rainbow trout were determined by DNA sequencing and PCR-single stranded conformation polymorphism (PCR-SSCP) methods. The assessment results demonstrated that these two site mutations are synonymous mutations and therefore 6 haplotypes were revealed in these samples (n = 120). Moreover, One way ANOVA of the SNPs and growth traits revealed the genotypes of SNP g.1904C > A are associated with the length and body weight at 90 and 150 days of age (P < 0.05), whereas no significant association was found between the SNP g.660T > C and growth trait parameters. These results provide evidence that SNPs in MSTN-1 gene may be associated with length and body weight during the early stages of life (from hatching to 5 months of age), therefore MSTN-1 gene may be used for rainbow trout breeding program.

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Agri Gene
Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
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期刊介绍: Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.
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