清除白细胞全血压缩输注过程中红细胞计数及血钾浓度变化的实验研究

Jin-Lian Cheng , Shi-Fan Han , Ya-Qin Li , Yin-Ping Chu , Yu-Mei Sun , Jin-Feng Guo
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引用次数: 1

摘要

目的观察全血压缩输注除白细胞过程中红细胞计数、形态及血钾的变化。方法制备人白细胞清除全血,并用不同规格的静脉留置针(18G、20G、22G、24G)连接输液器。观察血液从静脉留置针针尖端流出时不同压力(100mmhg、200mmhg、300mmhg)下红细胞计数和血清钾浓度。20G针取标本涂片于载玻片上,油浸透镜下观察红细胞形态学变化。结果不同压力、不同尺寸的静脉留置针在血液流经输液器时红细胞计数和血钾变化不大。此外,在普通光镜下观察血样,视野内粗刺细胞、长圆形细胞和纺锤形细胞计数随着压力的增加而逐渐增加。此外,一部分细胞发生了分裂。结论应用18G、20G、22G、24G静脉留置针在小于300mmhg压力下输血,短期内血液样本红细胞计数无明显变化。然而,有明显的红细胞形态学改变。研究结果可为保障输血患者的临床安全提供更多依据。
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An experimental study on RBC count and serum potassium concentration changes during compression transfusion of WBC-removal whole blood

Objective

To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood.

Methods

Prepared human WBC-removal whole blood and connected transfusion apparatus with different sizes of intravenous detaining needles (18G, 20G, 22G and 24G). Observed RBC count and serum potassium concentration under different pressures (100 mmHg, 200 mmHg, and 300 mmHg) as blood flowed out of the pinhead end of the intravenous detaining needle. Samples obtained with the 20G needle were smeared on glass slides, and RBC morphologic changes were observed under an oil immersion lens.

Results

RBC count and serum potassium changed slightly under different pressures with different sizes of intravenous detaining needles as blood flowed through the transfusion apparatus. In addition, the observation of blood samples under a common light microscope revealed that coarse-prick, oblong, and spindle cell counts in the visual fields increased gradually as the pressure increased. Additionally, a portion of cells had undergone splintering.

Conclusions

While applying 18G, 20G, 22G and 24G intravenous detaining needles for blood transfusion under less than 300 mmHg of pressure, no significant RBC count change was found in blood samples in the short term. However, there were significant RBC morphologic changes. The results could offer more basis to ensure the clinical safety of patients undergoing blood transfusion.

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