{"title":"下一代测序法对伊朗利什曼原虫前鞭毛体转录组测序的初步信息。","authors":"Farveh Ehya, Sima Kalantari, Mojgan Bandehpour, Bahram Kazemi","doi":"10.18502/ijpa.v18i3.13759","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>A lizard <i>Leishmania</i> has been isolated from a lizard (<i>Agama agilis</i>) in Iran. Its genome sequence has not been determined, so far.</p><p><strong>Methods: </strong>The study was done at Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2017-2023. <i>Leishmania</i> promastigotes were cultured in RPMI<sub>1640</sub> culture medium and collected at logarithmic phase by centrigugation. Parasite RNA was extracted by the Qiagene standard kit and its quantity and quality was determined and sequenced by NGS method with Illumina PE machine at BGI Company (China).</p><p><strong>Results: </strong>The number of 8316 mRNA, 83 tRNA, 63 rRNA, 83 ncRNA, 5 snRNA, 1039 snoRNA, 36 region, and 3 repeat regions, 8343 CDS, 9597 Exon and 9292 Genes were identified in promastigote of Iranian lizard <i>Leishmania</i>.</p><p><strong>Conclusion: </strong>Genomic elements of Iranian lizards <i>Leishmania</i> (with unique characteristics) were determined and identified by NGS system.</p>","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"18 3","pages":"362-368"},"PeriodicalIF":0.9000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e9/f8/IJPA-18-362.PMC10597888.pdf","citationCount":"0","resultStr":"{\"title\":\"Preliminary Information of Iranian Lizard <i>Leishmania</i> Promastigote Transcriptome Sequencing by Next Generation Sequencing (NGS) Method.\",\"authors\":\"Farveh Ehya, Sima Kalantari, Mojgan Bandehpour, Bahram Kazemi\",\"doi\":\"10.18502/ijpa.v18i3.13759\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>A lizard <i>Leishmania</i> has been isolated from a lizard (<i>Agama agilis</i>) in Iran. Its genome sequence has not been determined, so far.</p><p><strong>Methods: </strong>The study was done at Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2017-2023. <i>Leishmania</i> promastigotes were cultured in RPMI<sub>1640</sub> culture medium and collected at logarithmic phase by centrigugation. Parasite RNA was extracted by the Qiagene standard kit and its quantity and quality was determined and sequenced by NGS method with Illumina PE machine at BGI Company (China).</p><p><strong>Results: </strong>The number of 8316 mRNA, 83 tRNA, 63 rRNA, 83 ncRNA, 5 snRNA, 1039 snoRNA, 36 region, and 3 repeat regions, 8343 CDS, 9597 Exon and 9292 Genes were identified in promastigote of Iranian lizard <i>Leishmania</i>.</p><p><strong>Conclusion: </strong>Genomic elements of Iranian lizards <i>Leishmania</i> (with unique characteristics) were determined and identified by NGS system.</p>\",\"PeriodicalId\":14669,\"journal\":{\"name\":\"Iranian Journal of Parasitology\",\"volume\":\"18 3\",\"pages\":\"362-368\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e9/f8/IJPA-18-362.PMC10597888.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/ijpa.v18i3.13759\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/ijpa.v18i3.13759","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next Generation Sequencing (NGS) Method.
Background: A lizard Leishmania has been isolated from a lizard (Agama agilis) in Iran. Its genome sequence has not been determined, so far.
Methods: The study was done at Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2017-2023. Leishmania promastigotes were cultured in RPMI1640 culture medium and collected at logarithmic phase by centrigugation. Parasite RNA was extracted by the Qiagene standard kit and its quantity and quality was determined and sequenced by NGS method with Illumina PE machine at BGI Company (China).
Results: The number of 8316 mRNA, 83 tRNA, 63 rRNA, 83 ncRNA, 5 snRNA, 1039 snoRNA, 36 region, and 3 repeat regions, 8343 CDS, 9597 Exon and 9292 Genes were identified in promastigote of Iranian lizard Leishmania.
Conclusion: Genomic elements of Iranian lizards Leishmania (with unique characteristics) were determined and identified by NGS system.
期刊介绍：
Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members.
The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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