{"title":"两性偶氮染料比较荧光凝胶电泳(CoFGE)的pi控制","authors":"Marina Hanneken , Karel Šlais , Simone König","doi":"10.1016/j.euprot.2015.03.003","DOIUrl":null,"url":null,"abstract":"<div><p>Gel-to-gel variation is a major problem in two-dimensional (2D) gel electrophoresis. Vertical and horizontal comparative fluorescence gel electrophoresis (v/hCoFGE) were recently introduced to improve the reproducibility of protein spot assignment. There, in a combination of 1D- and 2D-PAGE, protein analytes were overlaid with a reference grid which allowed correcting for variation in the <em>y</em>-direction. However, control of the first dimension, p<em>I</em> (<em>x</em>-direction), was missing so far. Here, we applied amphoteric azo dyes for this purpose completing the CoFGE toolkit.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"8 ","pages":"Pages 36-39"},"PeriodicalIF":0.0000,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.03.003","citationCount":"4","resultStr":"{\"title\":\"pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes\",\"authors\":\"Marina Hanneken , Karel Šlais , Simone König\",\"doi\":\"10.1016/j.euprot.2015.03.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Gel-to-gel variation is a major problem in two-dimensional (2D) gel electrophoresis. Vertical and horizontal comparative fluorescence gel electrophoresis (v/hCoFGE) were recently introduced to improve the reproducibility of protein spot assignment. There, in a combination of 1D- and 2D-PAGE, protein analytes were overlaid with a reference grid which allowed correcting for variation in the <em>y</em>-direction. However, control of the first dimension, p<em>I</em> (<em>x</em>-direction), was missing so far. Here, we applied amphoteric azo dyes for this purpose completing the CoFGE toolkit.</p></div>\",\"PeriodicalId\":38260,\"journal\":{\"name\":\"EuPA Open Proteomics\",\"volume\":\"8 \",\"pages\":\"Pages 36-39\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.euprot.2015.03.003\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"EuPA Open Proteomics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212968515000094\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"EuPA Open Proteomics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212968515000094","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes
Gel-to-gel variation is a major problem in two-dimensional (2D) gel electrophoresis. Vertical and horizontal comparative fluorescence gel electrophoresis (v/hCoFGE) were recently introduced to improve the reproducibility of protein spot assignment. There, in a combination of 1D- and 2D-PAGE, protein analytes were overlaid with a reference grid which allowed correcting for variation in the y-direction. However, control of the first dimension, pI (x-direction), was missing so far. Here, we applied amphoteric azo dyes for this purpose completing the CoFGE toolkit.
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