M. Steinitz, Ilkka Seppälä, K. Eichmann , George Klein
{"title":"具有抗A群链球菌碳水化合物特异性抗体的人淋巴母细胞系的建立","authors":"M. Steinitz, Ilkka Seppälä, K. Eichmann , George Klein","doi":"10.1016/S0340-904X(79)80060-3","DOIUrl":null,"url":null,"abstract":"<div><p>A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosaminecoupled T<sub>4</sub>-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 I-lg/ml anti-A-CHO antibodies of the μgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.</p></div>","PeriodicalId":101288,"journal":{"name":"Zeitschrift für Immunit?tsforschung: Immunobiology","volume":"156 1","pages":"Pages 41-47"},"PeriodicalIF":0.0000,"publicationDate":"1979-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0340-904X(79)80060-3","citationCount":"22","resultStr":"{\"title\":\"Establishment of A Human Lymphoblastoid Cell Line with Specific Antibody Production Against Group A Streptococcal Carbohydrate\",\"authors\":\"M. Steinitz, Ilkka Seppälä, K. Eichmann , George Klein\",\"doi\":\"10.1016/S0340-904X(79)80060-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosaminecoupled T<sub>4</sub>-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 I-lg/ml anti-A-CHO antibodies of the μgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.</p></div>\",\"PeriodicalId\":101288,\"journal\":{\"name\":\"Zeitschrift für Immunit?tsforschung: Immunobiology\",\"volume\":\"156 1\",\"pages\":\"Pages 41-47\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1979-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0340-904X(79)80060-3\",\"citationCount\":\"22\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift für Immunit?tsforschung: Immunobiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0340904X79800603\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift für Immunit?tsforschung: Immunobiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0340904X79800603","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Establishment of A Human Lymphoblastoid Cell Line with Specific Antibody Production Against Group A Streptococcal Carbohydrate
A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosaminecoupled T4-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 I-lg/ml anti-A-CHO antibodies of the μgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.
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