Responsive microgels and microgel assemblies in biocatalytic applications

Microgels are soft particles that offer excellent colloidal stability, large surface area, and fluid-like transport characteristics, making them ideal for enzyme immobilization and reaction regulation. In recent years, the use of microgels in enzyme processes has rapidly increased and reviewed. Here in this review, we have provided a comprehensive overview of the strategies involved in microgel synthesis, practical aspects of microgel–enzyme conjugate formation, and the effects of microgel on enzyme stabilization and activities. In the end, we have discussed important biocatalytic applications of microgel and enzyme. The aim of this review is to provide a detailed understanding of enzyme–microgel conjugates and their potential applications, which can aid in the design of new microgels, responsive platforms, and biochemical applications.