{"title":"多元醇对脂肪酶热稳定性的影响及其在棉织物酶处理中的应用","authors":"K. Fu, Da-nian Lu","doi":"10.1080/00405000.2013.787240","DOIUrl":null,"url":null,"abstract":"In this paper, the effect of polyols on thermal stability of lipase was investigated. Lipase, with or without polyols was heated at 75°C for different periods of time. Half-life (t1/2, min) of lipase at 75°C was prolonged from 8.74 to 12.62, 11.66, 9.45, and 8.95 with the addition of d-sorbierite, d-fructose, mannite, and maltose, respectively. Conformational changes of lipase after the heat treatment were studied by both Fluorescence spectra test and UV spectra test. The UV values (as the unfolding ratios of lipase) calculated from UV spectra were related to the proportion of inactivated lipase. Residual activityUV (originated from UV values) reflected residual lipase activity from the experimental data accurately. Compared with cotton fabrics treated by the crude lipase at 75°C, the appearance of polyols enhanced the capillary effect and weight loss of cotton fabrics to some extent.","PeriodicalId":49978,"journal":{"name":"Journal of the Textile Institute","volume":"104 1","pages":"1206 - 1212"},"PeriodicalIF":1.5000,"publicationDate":"2013-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00405000.2013.787240","citationCount":"2","resultStr":"{\"title\":\"Polyols’ effect on thermal stability of lipase and its application in cotton fabrics’ enzymatic treatment\",\"authors\":\"K. Fu, Da-nian Lu\",\"doi\":\"10.1080/00405000.2013.787240\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this paper, the effect of polyols on thermal stability of lipase was investigated. Lipase, with or without polyols was heated at 75°C for different periods of time. Half-life (t1/2, min) of lipase at 75°C was prolonged from 8.74 to 12.62, 11.66, 9.45, and 8.95 with the addition of d-sorbierite, d-fructose, mannite, and maltose, respectively. Conformational changes of lipase after the heat treatment were studied by both Fluorescence spectra test and UV spectra test. The UV values (as the unfolding ratios of lipase) calculated from UV spectra were related to the proportion of inactivated lipase. Residual activityUV (originated from UV values) reflected residual lipase activity from the experimental data accurately. Compared with cotton fabrics treated by the crude lipase at 75°C, the appearance of polyols enhanced the capillary effect and weight loss of cotton fabrics to some extent.\",\"PeriodicalId\":49978,\"journal\":{\"name\":\"Journal of the Textile Institute\",\"volume\":\"104 1\",\"pages\":\"1206 - 1212\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2013-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/00405000.2013.787240\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Textile Institute\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://doi.org/10.1080/00405000.2013.787240\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, TEXTILES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Textile Institute","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1080/00405000.2013.787240","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, TEXTILES","Score":null,"Total":0}
Polyols’ effect on thermal stability of lipase and its application in cotton fabrics’ enzymatic treatment
In this paper, the effect of polyols on thermal stability of lipase was investigated. Lipase, with or without polyols was heated at 75°C for different periods of time. Half-life (t1/2, min) of lipase at 75°C was prolonged from 8.74 to 12.62, 11.66, 9.45, and 8.95 with the addition of d-sorbierite, d-fructose, mannite, and maltose, respectively. Conformational changes of lipase after the heat treatment were studied by both Fluorescence spectra test and UV spectra test. The UV values (as the unfolding ratios of lipase) calculated from UV spectra were related to the proportion of inactivated lipase. Residual activityUV (originated from UV values) reflected residual lipase activity from the experimental data accurately. Compared with cotton fabrics treated by the crude lipase at 75°C, the appearance of polyols enhanced the capillary effect and weight loss of cotton fabrics to some extent.
期刊介绍：
The Journal of The Textile Institute welcomes papers concerning research and innovation, reflecting the professional interests of the Textile Institute in science, engineering, economics, management and design related to the textile industry and the use of fibres in consumer and engineering applications. Papers may encompass anything in the range of textile activities, from fibre production through textile processes and machines, to the design, marketing and use of products. Papers may also report fundamental theoretical or experimental investigations, including materials science topics in nanotechnology and smart materials, practical or commercial industrial studies and may relate to technical, economic, aesthetic, social or historical aspects of textiles and the textile industry.
All published research articles in The Journal of The Textile Institute have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by two expert referees.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
