{"title":"母亲孕前体重指数和学龄前超重/肥胖的后代:脐带血表观基因组DNA甲基化变化的可能作用","authors":"Ruixia Chang, Yuanyuan Zhang, Jiahong Sun, Ke Xu, Chunan Li, Jingli Zhang, Wenhua Mei, Hongzhong Zhang, Jianduan Zhang","doi":"10.1111/ijpo.12969","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Epigenome-wide association studies have identified some DNA methylation sites associated with body mass index (BMI) or obesity. Studies in the Asian population are lacking.</p>\n </section>\n \n <section>\n \n <h3> Objective</h3>\n \n <p>To examine the association of cord blood genome-wide DNA methylation (GWDm) changes with maternal pre-pregnancy BMI and children's BMI-z score at preschool age. Additionally, we also explored the genome-wide differentially methylated regions and differentially methylated probes between preschoolers with overweight/obesity and normal-weight counterparts.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>This two-stage study design included (1) a GWDm analysis of 30 mother–child pairs from 633 participants of the Zhuhai birth cohort with data on newborn cord blood, maternal pre-pregnancy BMI, and children's BMI at 3 years of age; and (2) a targeted validation analysis of the cord blood of ten children with overweight/obesity and ten matched controls to validate the CpG sites.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>In the first stage, no significant CpG sites were found to be associated with children's BMI-z score at preschool age after <i>FDR</i> correction with the <i>p</i>-values of the CpG sites in <i>FOXN3</i> (cg23501836) and <i>ZNF264</i> (cg27437574) being close to 1 × 10<sup>−6</sup>. In the second stage, a significant difference of CpG sites in <i>AHRR</i> (chr5:355067-355068) and <i>FOXN3</i> (chr14: 89630264-89630272 and chr14: 89630387-89630388) was found between the ten children with overweight/obesity and ten controls (<i>p</i> < 0.05). The CpG sites in <i>FOXN3</i> (chr14:89630264-89630272 and chr14:89630295-89630296) and <i>ZNF264</i> (chr19: 57703104-57703107 and chr19: 57703301-57703307) were associated with children's BMI-z score; and the CpG sites in <i>FOXN3</i> (chr14: 89630264-89630272 and chr14: 89630387-89630388) were associated with maternal pre-pregnancy BMI.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>DNA methylation in <i>FOXN3</i> and <i>AHRR</i> is associated with overweight/obesity in preschool-aged children, and the methylation in <i>FOXN3</i> and <i>ZNF264</i> might be associated with children's BMI-z score. <i>FOXN3</i> methylation may be associated with maternal pre-pregnancy BMI, suggesting its potential role in the children's BMI-z score or overweight/obesity. Our results provide novel insights into the mechanisms of children's obesity.</p>\n </section>\n </div>","PeriodicalId":217,"journal":{"name":"Pediatric Obesity","volume":"18 1","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Maternal pre-pregnancy body mass index and offspring with overweight/obesity at preschool age: The possible role of epigenome-wide DNA methylation changes in cord blood\",\"authors\":\"Ruixia Chang, Yuanyuan Zhang, Jiahong Sun, Ke Xu, Chunan Li, Jingli Zhang, Wenhua Mei, Hongzhong Zhang, Jianduan Zhang\",\"doi\":\"10.1111/ijpo.12969\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Epigenome-wide association studies have identified some DNA methylation sites associated with body mass index (BMI) or obesity. Studies in the Asian population are lacking.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>To examine the association of cord blood genome-wide DNA methylation (GWDm) changes with maternal pre-pregnancy BMI and children's BMI-z score at preschool age. Additionally, we also explored the genome-wide differentially methylated regions and differentially methylated probes between preschoolers with overweight/obesity and normal-weight counterparts.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>This two-stage study design included (1) a GWDm analysis of 30 mother–child pairs from 633 participants of the Zhuhai birth cohort with data on newborn cord blood, maternal pre-pregnancy BMI, and children's BMI at 3 years of age; and (2) a targeted validation analysis of the cord blood of ten children with overweight/obesity and ten matched controls to validate the CpG sites.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>In the first stage, no significant CpG sites were found to be associated with children's BMI-z score at preschool age after <i>FDR</i> correction with the <i>p</i>-values of the CpG sites in <i>FOXN3</i> (cg23501836) and <i>ZNF264</i> (cg27437574) being close to 1 × 10<sup>−6</sup>. In the second stage, a significant difference of CpG sites in <i>AHRR</i> (chr5:355067-355068) and <i>FOXN3</i> (chr14: 89630264-89630272 and chr14: 89630387-89630388) was found between the ten children with overweight/obesity and ten controls (<i>p</i> < 0.05). The CpG sites in <i>FOXN3</i> (chr14:89630264-89630272 and chr14:89630295-89630296) and <i>ZNF264</i> (chr19: 57703104-57703107 and chr19: 57703301-57703307) were associated with children's BMI-z score; and the CpG sites in <i>FOXN3</i> (chr14: 89630264-89630272 and chr14: 89630387-89630388) were associated with maternal pre-pregnancy BMI.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>DNA methylation in <i>FOXN3</i> and <i>AHRR</i> is associated with overweight/obesity in preschool-aged children, and the methylation in <i>FOXN3</i> and <i>ZNF264</i> might be associated with children's BMI-z score. <i>FOXN3</i> methylation may be associated with maternal pre-pregnancy BMI, suggesting its potential role in the children's BMI-z score or overweight/obesity. Our results provide novel insights into the mechanisms of children's obesity.</p>\\n </section>\\n </div>\",\"PeriodicalId\":217,\"journal\":{\"name\":\"Pediatric Obesity\",\"volume\":\"18 1\",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2022-09-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pediatric Obesity\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/ijpo.12969\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PEDIATRICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pediatric Obesity","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ijpo.12969","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PEDIATRICS","Score":null,"Total":0}
Maternal pre-pregnancy body mass index and offspring with overweight/obesity at preschool age: The possible role of epigenome-wide DNA methylation changes in cord blood
Background
Epigenome-wide association studies have identified some DNA methylation sites associated with body mass index (BMI) or obesity. Studies in the Asian population are lacking.
Objective
To examine the association of cord blood genome-wide DNA methylation (GWDm) changes with maternal pre-pregnancy BMI and children's BMI-z score at preschool age. Additionally, we also explored the genome-wide differentially methylated regions and differentially methylated probes between preschoolers with overweight/obesity and normal-weight counterparts.
Methods
This two-stage study design included (1) a GWDm analysis of 30 mother–child pairs from 633 participants of the Zhuhai birth cohort with data on newborn cord blood, maternal pre-pregnancy BMI, and children's BMI at 3 years of age; and (2) a targeted validation analysis of the cord blood of ten children with overweight/obesity and ten matched controls to validate the CpG sites.
Results
In the first stage, no significant CpG sites were found to be associated with children's BMI-z score at preschool age after FDR correction with the p-values of the CpG sites in FOXN3 (cg23501836) and ZNF264 (cg27437574) being close to 1 × 10−6. In the second stage, a significant difference of CpG sites in AHRR (chr5:355067-355068) and FOXN3 (chr14: 89630264-89630272 and chr14: 89630387-89630388) was found between the ten children with overweight/obesity and ten controls (p < 0.05). The CpG sites in FOXN3 (chr14:89630264-89630272 and chr14:89630295-89630296) and ZNF264 (chr19: 57703104-57703107 and chr19: 57703301-57703307) were associated with children's BMI-z score; and the CpG sites in FOXN3 (chr14: 89630264-89630272 and chr14: 89630387-89630388) were associated with maternal pre-pregnancy BMI.
Conclusions
DNA methylation in FOXN3 and AHRR is associated with overweight/obesity in preschool-aged children, and the methylation in FOXN3 and ZNF264 might be associated with children's BMI-z score. FOXN3 methylation may be associated with maternal pre-pregnancy BMI, suggesting its potential role in the children's BMI-z score or overweight/obesity. Our results provide novel insights into the mechanisms of children's obesity.
期刊介绍：
Pediatric Obesity is a peer-reviewed, monthly journal devoted to research into obesity during childhood and adolescence. The topic is currently at the centre of intense interest in the scientific community, and is of increasing concern to health policy-makers and the public at large.
Pediatric Obesity has established itself as the leading journal for high quality papers in this field, including, but not limited to, the following:
Genetic, molecular, biochemical and physiological aspects of obesity – basic, applied and clinical studies relating to mechanisms of the development of obesity throughout the life course and the consequent effects of obesity on health outcomes
Metabolic consequences of child and adolescent obesity
Epidemiological and population-based studies of child and adolescent overweight and obesity
Measurement and diagnostic issues in assessing child and adolescent adiposity, physical activity and nutrition
Clinical management of children and adolescents with obesity including studies of treatment and prevention
Co-morbidities linked to child and adolescent obesity – mechanisms, assessment, and treatment
Life-cycle factors eg familial, intrauterine and developmental aspects of child and adolescent obesity
Nutrition security and the "double burden" of obesity and malnutrition
Health promotion strategies around the issues of obesity, nutrition and physical activity in children and adolescents
Community and public health measures to prevent overweight and obesity in children and adolescents.
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