{"title":"无血清细胞培养制备抗人IgA单克隆抗体","authors":"Katsuhik Sugino, S. Kobatake, S. Matsuura","doi":"10.14921/JSCC1971B.19.1_31","DOIUrl":null,"url":null,"abstract":"A hybridoma secreting monoclonal anti-human IgA antibody was subcloned and adapted to serum serum-free medium. The cells have stably grown and produced an antibody. The monoclonal ntibody (MoAb) thus produced in this serum-free medium was simply purified by precipitation. Characteristics of the antibody was comparable to that obtained from mouse ascitic tumor.It was also demonstrated to be utilized in an automated turbidimetric assay. MoAbs produced by serum-free Cell culture system may offer great potential for the immunodiagnostic reagents.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1990-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An Anti-Human IgA Monoclonal Antibody Produced by Serum-Free Cell Culture\",\"authors\":\"Katsuhik Sugino, S. Kobatake, S. Matsuura\",\"doi\":\"10.14921/JSCC1971B.19.1_31\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A hybridoma secreting monoclonal anti-human IgA antibody was subcloned and adapted to serum serum-free medium. The cells have stably grown and produced an antibody. The monoclonal ntibody (MoAb) thus produced in this serum-free medium was simply purified by precipitation. Characteristics of the antibody was comparable to that obtained from mouse ascitic tumor.It was also demonstrated to be utilized in an automated turbidimetric assay. MoAbs produced by serum-free Cell culture system may offer great potential for the immunodiagnostic reagents.\",\"PeriodicalId\":39360,\"journal\":{\"name\":\"Japanese Journal of Clinical Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-03-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese Journal of Clinical Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14921/JSCC1971B.19.1_31\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Clinical Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14921/JSCC1971B.19.1_31","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
An Anti-Human IgA Monoclonal Antibody Produced by Serum-Free Cell Culture
A hybridoma secreting monoclonal anti-human IgA antibody was subcloned and adapted to serum serum-free medium. The cells have stably grown and produced an antibody. The monoclonal ntibody (MoAb) thus produced in this serum-free medium was simply purified by precipitation. Characteristics of the antibody was comparable to that obtained from mouse ascitic tumor.It was also demonstrated to be utilized in an automated turbidimetric assay. MoAbs produced by serum-free Cell culture system may offer great potential for the immunodiagnostic reagents.
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