{"title":"mutR基因在emm12型链球菌菌株的新陈代谢和毒性方面的作用","authors":"A. A. Zutkis, B. Milman, A. Dmitriev","doi":"10.15789/2220-7619-2014-4-339-346","DOIUrl":null,"url":null,"abstract":"In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis. Inactivation of the mutR gene was found to affect the dynamic and characteristics of bacterial growth in liquid medium. Expression of secreted nucleases was significantly lower in the mutant strains compared to the wild-type strains. Two-dimensional electrophoresis and mass-spectrometry revealed differences in expression of number of the proteins in mutant strains compared to the wild-type strains. Inactivation of the mutR gene negatively affected capacity of S. pyogenes to adhere to human epithelial cells. Finally, the virulence properties of the no. 152[mutR] mutant strains were found to be 4,7-fold less compared to the strain no. 152, while the no. 97[mutR] mutant strain became avirulent compared to the strain no. 97 due to insertional inactivation of the mutR gene.","PeriodicalId":42907,"journal":{"name":"Infektsiya i Immunitet","volume":"4 1","pages":"339-346"},"PeriodicalIF":0.2000,"publicationDate":"2015-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"РОЛЬ ГЕНА mutR В МЕТАБОЛИЗМЕ И ВИРУЛЕНТНОСТИ ШТАММОВ STREPTOCOCCUS PYOGENES ГЕНОТИПА emm12\",\"authors\":\"A. A. Zutkis, B. Milman, A. Dmitriev\",\"doi\":\"10.15789/2220-7619-2014-4-339-346\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis. Inactivation of the mutR gene was found to affect the dynamic and characteristics of bacterial growth in liquid medium. Expression of secreted nucleases was significantly lower in the mutant strains compared to the wild-type strains. Two-dimensional electrophoresis and mass-spectrometry revealed differences in expression of number of the proteins in mutant strains compared to the wild-type strains. Inactivation of the mutR gene negatively affected capacity of S. pyogenes to adhere to human epithelial cells. Finally, the virulence properties of the no. 152[mutR] mutant strains were found to be 4,7-fold less compared to the strain no. 152, while the no. 97[mutR] mutant strain became avirulent compared to the strain no. 97 due to insertional inactivation of the mutR gene.\",\"PeriodicalId\":42907,\"journal\":{\"name\":\"Infektsiya i Immunitet\",\"volume\":\"4 1\",\"pages\":\"339-346\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2015-02-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infektsiya i Immunitet\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15789/2220-7619-2014-4-339-346\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infektsiya i Immunitet","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15789/2220-7619-2014-4-339-346","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
РОЛЬ ГЕНА mutR В МЕТАБОЛИЗМЕ И ВИРУЛЕНТНОСТИ ШТАММОВ STREPTOCOCCUS PYOGENES ГЕНОТИПА emm12
In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis. Inactivation of the mutR gene was found to affect the dynamic and characteristics of bacterial growth in liquid medium. Expression of secreted nucleases was significantly lower in the mutant strains compared to the wild-type strains. Two-dimensional electrophoresis and mass-spectrometry revealed differences in expression of number of the proteins in mutant strains compared to the wild-type strains. Inactivation of the mutR gene negatively affected capacity of S. pyogenes to adhere to human epithelial cells. Finally, the virulence properties of the no. 152[mutR] mutant strains were found to be 4,7-fold less compared to the strain no. 152, while the no. 97[mutR] mutant strain became avirulent compared to the strain no. 97 due to insertional inactivation of the mutR gene.
期刊介绍：
Journal "Infektsiya i immunitet" ("Russian Journal of Infection and Immunity") established by Northwest Branch of RAMS, St. Petersburg Pasteur Institute and the St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists, with the participation of the St. Petersburg branch of All-Russian Practical Society of epidemiologists, microbiologists and parasitologists at St. Petersburg and Leningrad region. The journal is devoted to numerous aspects of the interaction between different microorganisms and the host organism. Journal is of interest for microbiologists, immunologists, epidemiologists and clinicians. The most detailed discussion of the following questions: • molecular basis of infections caused by pathogenic bacteria, fungi and parasites; • mechanisms of pathogenicity of microorganisms; • the impact of microbial virulence factors on host cells; • factors and mechanism to protect the host from infection; • factors of nonspecific and specific immunity; • experimental models of infectious disease; • development of vaccines and nonspecific anti-infectious defense.
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