自动测量DNA断裂数的图像处理方法

IF 0.4 Q4 ENGINEERING, MULTIDISCIPLINARY Journal of Advanced Simulation in Science and Engineering Pub Date : 2021-01-01 DOI:10.15748/jasse.8.173
S. Saito, Hiroaki Nakamura, T. Kenmotsu, Y. Oya, Y. Hatano, Y. Tamura, Susumu Fujiwara, H. Ohtani
{"title":"自动测量DNA断裂数的图像处理方法","authors":"S. Saito, Hiroaki Nakamura, T. Kenmotsu, Y. Oya, Y. Hatano, Y. Tamura, Susumu Fujiwara, H. Ohtani","doi":"10.15748/jasse.8.173","DOIUrl":null,"url":null,"abstract":". The number of double - strand breaks can be evaluated from the change of average DNA length. The average DNA length is measured by the single - molecule observation method using fluorescence microscope. The measurement of DNA length in the microscope images is done manually by experienced operators and it is time consuming in many experi-ments. An image processing method using OpenCV library to measure length of DNA in fluorescence microscope images is developed in this paper. An automation of measurement using deep learning is also proposed.","PeriodicalId":41942,"journal":{"name":"Journal of Advanced Simulation in Science and Engineering","volume":"1 1","pages":""},"PeriodicalIF":0.4000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Image processing method for automatic measurement of number of DNA breaks\",\"authors\":\"S. Saito, Hiroaki Nakamura, T. Kenmotsu, Y. Oya, Y. Hatano, Y. Tamura, Susumu Fujiwara, H. Ohtani\",\"doi\":\"10.15748/jasse.8.173\",\"DOIUrl\":null,\"url\":null,\"abstract\":\". The number of double - strand breaks can be evaluated from the change of average DNA length. The average DNA length is measured by the single - molecule observation method using fluorescence microscope. The measurement of DNA length in the microscope images is done manually by experienced operators and it is time consuming in many experi-ments. An image processing method using OpenCV library to measure length of DNA in fluorescence microscope images is developed in this paper. An automation of measurement using deep learning is also proposed.\",\"PeriodicalId\":41942,\"journal\":{\"name\":\"Journal of Advanced Simulation in Science and Engineering\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Advanced Simulation in Science and Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15748/jasse.8.173\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENGINEERING, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Simulation in Science and Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15748/jasse.8.173","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

. 双链断裂的数量可以通过DNA平均长度的变化来判断。采用荧光显微镜单分子观察法测定平均DNA长度。显微镜图像中DNA长度的测量是由经验丰富的操作人员手工完成的,并且在许多实验中耗时。本文提出了一种利用OpenCV文库测量荧光显微镜图像中DNA长度的图像处理方法。提出了一种利用深度学习实现测量自动化的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Image processing method for automatic measurement of number of DNA breaks
. The number of double - strand breaks can be evaluated from the change of average DNA length. The average DNA length is measured by the single - molecule observation method using fluorescence microscope. The measurement of DNA length in the microscope images is done manually by experienced operators and it is time consuming in many experi-ments. An image processing method using OpenCV library to measure length of DNA in fluorescence microscope images is developed in this paper. An automation of measurement using deep learning is also proposed.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
17
期刊最新文献
Implementation and performance evaluation of a hierarchical parallel solver for saddle point problems on a GPU cluster Molecular Dynamics Simulation on Hydrogen Trapping on Tungsten Vacancy Fundamental study on magnetohydrodynamic simulation method using deep learning Semi-explicit large eddy simulation in non-reacting air/gas fuel jet flows A human motion angle prediction method using a neural network and feature-extraction-processed sEMG obtained in real time to utilize mechanical delay of sEMG
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1