Girish Tigari, J. G. Manjunatha, D. Ravishankar, G. Siddaraju
{"title":"聚精氨酸修饰碳糊电极强化电化学测定生物和药物样品中的核黄素","authors":"Girish Tigari, J. G. Manjunatha, D. Ravishankar, G. Siddaraju","doi":"10.17721/moca.2019.216-223","DOIUrl":null,"url":null,"abstract":"An electrogenerated Polyarginine modified carbon paste electrode (PAMCPE) was fabricated through a simple electropolymerization procedure. The devised electrode was characterized by cyclic voltammetry (CV) and Field Emission Scanning Electron Microscopy (FESEM). This electrode was utilized for electrocatalytic estimation of Riboflavin (RF) and its instantaneous resolution with ascorbic acid (AA) and folic acid (FA) in phosphate buffer solution (PBS) of pH 6.0 by differential pulse voltammetry (DPV). It was observed to be a very responsive electrode for the electrochemical detection and quantification of RF. It was revealed that PAMCPE generates higher current response towards RF contrast to the bare carbon paste electrode (BCPE). Under optimized condition, the RF oxidation current values were linearly reliant on the RF concentration increment with a limit of detection (LOD) of 9.3·10-8 M using DPV. The stable PAMCPE was effectively applied for estimation of RF in B-complex pill and complex human blood serum samples.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"Enhanced Electrochemical Determination Of Riboflavin In Biological And Pharmaceutical Samples At Poly (Arginine) Modified Carbon Paste Electrode\",\"authors\":\"Girish Tigari, J. G. Manjunatha, D. Ravishankar, G. Siddaraju\",\"doi\":\"10.17721/moca.2019.216-223\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"An electrogenerated Polyarginine modified carbon paste electrode (PAMCPE) was fabricated through a simple electropolymerization procedure. The devised electrode was characterized by cyclic voltammetry (CV) and Field Emission Scanning Electron Microscopy (FESEM). This electrode was utilized for electrocatalytic estimation of Riboflavin (RF) and its instantaneous resolution with ascorbic acid (AA) and folic acid (FA) in phosphate buffer solution (PBS) of pH 6.0 by differential pulse voltammetry (DPV). It was observed to be a very responsive electrode for the electrochemical detection and quantification of RF. It was revealed that PAMCPE generates higher current response towards RF contrast to the bare carbon paste electrode (BCPE). Under optimized condition, the RF oxidation current values were linearly reliant on the RF concentration increment with a limit of detection (LOD) of 9.3·10-8 M using DPV. The stable PAMCPE was effectively applied for estimation of RF in B-complex pill and complex human blood serum samples.\",\"PeriodicalId\":18626,\"journal\":{\"name\":\"Methods and Objects of Chemical Analysis\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Methods and Objects of Chemical Analysis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17721/moca.2019.216-223\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods and Objects of Chemical Analysis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17721/moca.2019.216-223","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Enhanced Electrochemical Determination Of Riboflavin In Biological And Pharmaceutical Samples At Poly (Arginine) Modified Carbon Paste Electrode
An electrogenerated Polyarginine modified carbon paste electrode (PAMCPE) was fabricated through a simple electropolymerization procedure. The devised electrode was characterized by cyclic voltammetry (CV) and Field Emission Scanning Electron Microscopy (FESEM). This electrode was utilized for electrocatalytic estimation of Riboflavin (RF) and its instantaneous resolution with ascorbic acid (AA) and folic acid (FA) in phosphate buffer solution (PBS) of pH 6.0 by differential pulse voltammetry (DPV). It was observed to be a very responsive electrode for the electrochemical detection and quantification of RF. It was revealed that PAMCPE generates higher current response towards RF contrast to the bare carbon paste electrode (BCPE). Under optimized condition, the RF oxidation current values were linearly reliant on the RF concentration increment with a limit of detection (LOD) of 9.3·10-8 M using DPV. The stable PAMCPE was effectively applied for estimation of RF in B-complex pill and complex human blood serum samples.
期刊介绍:
The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.