体外器官发生是黄檀繁殖的有效方法

IF 0.7 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pesquisa Agropecuaria Brasileira Pub Date : 2022-01-01 DOI:10.1590/s1678-3921.pab2022.v57.02766

Ingridh Medeiros Simões, C. P. D. Araujo, T. Mello, Thuanny Lins Monteiro Rosa, Natasha Vieira de Oliveira, M. Caldeira, E. R. Schmildt, J. Lopes, W. Otoni, R. S. Alexandre

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引用次数: 0

摘要

摘要本研究旨在建立一种有效的黄檀体外器官发生机制。为此，在30天的幼苗子叶节和节段进行切片。这些材料在含有6-苄基氨基嘌呤和硫代脲细胞分裂素不同组合的培养基中培养。在温度为27℃、光周期为16小时的生长室中培养60天后，分析其生长特性。无论是否添加细胞分裂素，子叶节的外植体表现出更大的形态发生潜力。在添加2.22µmol L-1 BAP的培养基中，子叶节段的离体繁殖效果最佳。

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In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra

Abstract The objective of this work was to establish an efficient protocol for the in vitro organogenesis of Dalbergia nigra. For this purpose, 30-day-old seedlings were sectioned at their cotyledonary nodes and nodal segments. These materials were cultivated in a medium with different combinations of the 6-benzylaminopurine and thidiazuron cytokinins. After 60 days in a growth chamber set at 27°C and a 16-hour photoperiod, growth characteristics were analyzed. Explants from the cotyledonary nodes show a greater morphogenetic potential, regardless of the addition of cytokinins. Cotyledonary nodes in the medium supplemented with 2.22 µmol L-1 BAP show the best combination for the in vitro propagation of D. nigra.

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来源期刊

Pesquisa Agropecuaria Brasileira 农林科学-农业综合

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

9-18 weeks

期刊介绍： Pesquisa Agropecuária Brasileira – PAB – is issued monthly by Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA, affiliated to Ministry of Agriculture, Livestock and Food Supply. PAB publishes original scientific-technological articles on Plant Physiology, Plant Pathology, Crop Science, Genetics, Soil Science, Food Technology and Animal Science. Its abbreviated title is Pesq. agropec. bras., and it should be used in bibliographies, footnotes, references and bibliographic strips.