向日葵叶的粗枝分离和鉴定。色谱仪非常薄

Roihatul Muti’ah, E. K. Hayati, Yani Triastutik
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引用次数: 1

摘要

本研究采用薄层色谱法对太阳花(Helianthus annuus L.)叶片粗提物esquit萜进行分离鉴定。用溶剂甲醇浸渍太阳花叶。然后用乙酸乙酯和正己烷溶剂进行液体萃取。乙酸乙酯萃取部位和正己烷萃取部位进行植物化学试验。两种提取物经化学试剂检测后,采用薄层色谱法进行倍半萜含量鉴定。乙酸乙酯萃取部位植物化学试验结果为正萜类、倍半萜和三萜,正己烷萃取部位为正萜类、倍半萜和甾体。所有的半萜烯与洗脱二氯甲烷:乙酸乙酯(4,8:0,2)显示为紫红色。乙酸乙酯萃取部位均含有半萜,Rf值为0.89;0.94和0.96。而正己烷萃取部分,所得洗脱物为六萜Rf 0.49;0.8和0.99。
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PEMISAHAN DAN IDENTIFIKASI EKSTRAK KASAR SESKUITERPEN DAUN BUNGA MATAHARI (Helianyhus annuus L.) DENGAN KROMATOGRAFI LAPIS TIPIS
The purpose of this research wasto separate and identify the leaf crude extracts esquiterpene of Sun flower ( Helianthus annuus L.) using thin layer chromatography. Sun flower leaf maceration method performed with the solvent methanol. Then performed liquid extraction with ethyl acetate and n-hexane solvent. Ethyl acetate extract fraction and n-hexane extract fraction furth erphyto chemical test. After being test edphyto chemical with reagents, both extracts was followed by sesquiterpene content identification using thin layer chromatography (TLC)  analytic. Phytochemical test result from ethyl acetate extract fraction was positive terpenoid, sesquiterpene and triterpene, while n-hexane extractfraction positive terpenoid, sesquiterpene andsteroid. All egedsesquiterpene with eluentdichloromethane: ethyl acetate (4,8:0,2) is shown with apurplestain. In the ethyl acetate extract fraction all egedsesquiterpene having Rf values of 0.89; 0.94, and 0.96. While n-hexane extract fraction, the resulting eluental legedsesquiterpene Rf 0.49; 0.8,and 0.99.
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审稿时长
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