Evandro Keller, G. Koch, Mayara Catherine Candido Silva, P. Wyler, J. C. Barbosa, J. Rezende, H. Duarte, R. Calegario
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引用次数: 0
摘要
摘要:在过去的几十年里,白蝇传播的高发病率病毒已经成为番茄田的一个问题,最近威胁到马铃薯作物。本研究于2015年至2018年在巴西帕拉纳州Araucária、Campo do Tenente、Campo Largo、Contenda、Lapa、Faxinal、Morretes、Reserva、Castro、Palmeira和 o o Mateus do Sul等市对番茄和马铃薯作物中的begomovirus和crinivirus进行了调查。提取叶片总DNA和RNA作为模板,分别用PCR和RT-PCR检测begomovirus和crinivirus。在215份番茄样品中,14份来自Faxinal。其余番茄样品和243份马铃薯样品均呈begomovirus和crinivirus阴性。结果表明,该病毒侵染帕南州番茄作物的发生率较低(6.5%),其中一个扩增片段的核苷酸序列与番茄失绿病毒(ToCV)同源性达99.71%。
Incidence of Begomovirus and Crinivirus in tomato and potato crops in Paraná State, Brazil
ABSTRACT: In the last decades, the high incidence of viruses transmitted by whiteflies has become a problem in the tomato fields, threatening, more recently, the potato crops. The present study carried out a survey of begomoviruses and criniviruses in tomato and potato crops, from 2015 to 2018, in the municipalities of Araucária, Campo do Tenente, Campo Largo, Contenda, Lapa, Faxinal, Morretes, Reserva, Castro, Palmeira and São Mateus do Sul, in Paraná State, Brazil. Total DNA and RNA from leaves were extracted and used as templates to detect, respectively, begomoviruses by PCR and criniviruses by RT-PCR. Out of 215 tomato samples, 14 from Faxinal were infected by crinivirus. The other tomato samples and 243 potato samples showed negative results for begomovirus and crinivirus. Results indicated a low incidence (6.5%) of crinivirus infecting tomato crops in Paraná State, and the nucleotide sequence of one amplified fragment shared 99.71% identity with tomato chlorotic virus (ToCV).
期刊介绍:
The purpose of Ciência Rural is to publish the results of original research, note and reviews which contribute significantly to knowledge in Agricultural Sciences. Preference will be given to original articles that develop news concepts or experimental approaches and are not merely repositories of scientific data. The decison of acceptance for publication lies with the Editors and is based on the recommendations of Editorial Comission, Area Committee and/ or ad hoc reviewers. The editors and reviewers are external to the institution.