PEMBERIAN PASAK BUMI MEMENGARUHI KADAR TESTOSTERON DAN SPERMATOZOA KAMBING PERANAKAN ETAWA

H. Pratomo, Y. Yudi
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引用次数: 2

摘要

摘要本研究旨在探讨长叶Eurycoma longifolia Jack对Etawa杂交山羊睾丸激素水平和精子质量的影响。山羊分为两组,每组3只。1组(K1)为对照组,每天早上9点口服蒸馏水20 ml,连续6 d; 2组(K2)给予长叶Eurycoma,剂量为90 mg/kg bw,加入蒸馏水20 ml,每天早上9点口服,连续6 d。在第1、3、6天采用酶联免疫吸附法(ELISA)检测睾酮浓度。精子质量包括活精子的浓度和百分比。结果表明,K2上睾酮水平从第1天(9.36 ng/ml)到第6天(12.43 ng/ml)逐渐升高(P<0.05)。在第3 ~ 6天,K2处理的活精率分别为88.6%和89.8%,而K2处理的活精率分别为91.0和92.7%。综上所述,在20 ml蒸馏水中添加90 mg/kg bw的巴沙米6 d,可提高埃塔瓦杂交山羊的睾酮水平和活精子率。
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PEMBERIAN PASAK BUMI MEMENGARUHI KADAR TESTOSTERON DAN SPERMATOZOA KAMBING PERANAKAN ETAWA (The Administration of Eurycoma longifolia Jack Affect the Level of Testosterone and Spermatozoa of Etawa Crossbreed Goat)
ABSTRACT The study aimed to find out the effect of Eurycoma longifolia Jack on testosterone level and spermatozoa quality of Etawa crossbreed goat. Goats were divided into two treatment groups, three goats each. Group 1 (K1) was control group which given orally 20 ml of distilled water every morning at 9:00 am for six days, and group 2 (K2) was administered with Eurycoma longifolia with the dose of 90 mg/kg bw in 20 ml of distilled water orally every morning at 9:00 am for six days. The concentration of testosterone was measured on day 1, 3, and 6 using the method of enzyme-linked immunosorbent assay (ELISA). The quality of sperm consisted of concentration and percentage of life sperm. The results showed that level of testosterone on K2 increased started from day 1 (9.36 ng/ml) to day 6 (12.43 ng/ml) (P<0.05). The percentage of life spermatozoa increased higher on K2 compare to K1 on day 3 to day 6, that was 88.6 and 89.8% on K1 to 91.0 and 92.7% on K2. In conclusion, the administration of pasak bumi with the dose of 90 mg/kg bw in 20 ml of distilled water for 6 days able to increase testosterone levels and percentage of life sperm of Etawa crossbreed goat.
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