柑桔体细胞胚发生植株和接穗砧木的生长性能

Q4 Agricultural and Biological Sciences Indonesian Journal of Agricultural Science Pub Date : 2014-10-13 DOI:10.21082/ijas.v15n2.2014.p71-78
N. F. Devy, Y. Yenni, Hardiyanto Hardiyanto
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引用次数: 2

摘要

柑桔愈伤组织体外培养的体细胞胚发生是柑桔获得游离病毒和遗传真型植株的一种繁殖方式。为了诱导该技术衍生的植株生长,应将其离体嫁接到柑橘砧木上。本研究旨在评价柑橘属植物cv的生长性能。以植株和接穗为砧木的Siam Kintamani (Citrus nobilis L.)。研究于2011年6月至2012年12月在印度尼西亚柑橘和亚热带水果研究所Tlekung研究站进行。这些处理是在苗圃里进行的,通过嫁接一株植物并在一株8个月大的日本雪铁龙(JC)砧木上发芽。一年生嫁接和出芽植株在苗圃养护后移栽到田间。田间研究采用3个重复的随机区组设计,以15株植物为单位样本。结果表明:在苗圃处理10 ~ 12个月后,由SE和嫁接植株衍生的暹罗金塔马尼幼苗的营养生长速度快于出芽植株。在田间,SE嫁接植株的生长仅在移栽后6个月有显著差异。SE离体繁殖植株可作为柑桔健康植株的良好替代砧木材料。因此,尤其在品种利用、生殖生长和大苗生产等方面还需进一步研究。
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THE GROWTH PERFORMANCE OF CITRUS DERIVED FROM SOMATIC EMBRYOGENESIS PLANTLET AND SCION STOCK
Somatic embryogenesis (SE) of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L.) that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC) rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.
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来源期刊
Indonesian Journal of Agricultural Science
Indonesian Journal of Agricultural Science Agricultural and Biological Sciences-Soil Science
CiteScore
1.00
自引率
0.00%
发文量
5
审稿时长
12 weeks
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