短臂离心模拟人工重力对先天性免疫tlr相关基因表达的影响

Q4 Biochemistry, Genetics and Molecular Biology Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine Pub Date : 2023-01-01 DOI:10.21687/0233-528x-2023-57-2-20-26

D. D. Vlasova, A. Sadova, N. S. Germanov, V. Galina, V. A. Shmarov, O. Kutko, M. Rykova, E. Antropova, M. I. Koloteva, E. Lysenko, K. Orlova, S. Ponomarev

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引用次数: 0

摘要

在这里，我们报道了tlr相关基因、NF-kB、AP-1和TLR4通路基因、凋亡蛋白、细胞因子和趋化因子编码基因在短臂离心机旋转后的表达变化。这项研究涉及10名年龄在25-40岁之间、几乎健康的男性志愿者。在人工重力作用前后分别从受试者血液中分离出CD14+单核细胞。细胞与TLR1-9配体孵育24小时，以评估单核细胞的储备潜能。实时荧光定量PCR检测基因表达。综上所述，旋转短臂离心机未发现对先天免疫tlr相关分支功能的负面影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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THE IMPACT OF ARTIFICIAL GRAVITY MODELLED IN SHORT-ARM CENTRIFUGE ON THE EXPRESSION OF TLR-ASSOCIATED GENES OF INNATE IMMUNITY

Here, we report the changes in the expression of TLR-associated genes, genes of NF-kB, AP-1, and TLR4 pathways, genes coding for apoptotic proteins, cytokines, and chemokines, after rotation on the short-arm centrifuge. The study involved 10 almost healthy male volunteers aged 25-40. CD14+ monocytes were isolated from the testers’ blood before and after the exposure to the artificial gravity. The cells were incubated with TLR1-9 ligands for 24 hours to assess the monocytes’ reserve potential. The gene expression was evaluated by real-time PCR. In sum, no negative effect of rotation on the short-arm centrifuge on the functioning of TLR-associated branch of innate immunity was detected.

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Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

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0.60

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0.00%

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