利用SCoT分子标记鉴定锦葵属植物的种类及遗传多样性

4区 农林科学 Q3 Agricultural and Biological Sciences Genetika-Belgrade Pub Date : 2022-01-01 DOI:10.2298/gensr2201369s
Yanxia Sun, Hanxiu Jiang, Fang Zeng, X. Pan, Xiaoxia Wu, Yue Qi, Xiaoyong Wu
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引用次数: 1

摘要

Alcea属是锦葵科的一员,是地中海多年生草本植物,主要分布在中东和西地中海盆地。Alcea是一个独特的属,有几个特有的分类群,其中许多来自伊朗-图兰植物地理区域的特有分类群特别有趣。该属在伊朗有34种,其中15种是特有的。鉴于藻属植物的重要性,本研究收集了藻属植物的分子数据。本研究使用的植物共83种,取材于分布于6个省的5种植物。5条引物扩增得到75条条带,其中66条为多态性条带,占88.12%。较高的平均MI值和PIC值表明SCoT引物在Alcea种间具有较高的多态性位点检测能力。所收集的5种间的遗传相似性在0.77 ~ 0.89之间。SCoT标记分析结果显示,角藻与紫藻的相似性最小,而紫藻与硫藻的相似性最大。目前的研究?本研究的目的是:1)是否有可能通过SCoT标记来鉴定Alcea的物种,2)这些物种在伊朗有什么遗传结构,3)物种间的相互关系是什么?目前的研究发现,SCoT标记可以用来识别物种。
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Species identification and genetic diversity of Alcea (Malvaceae) using SCoT molecular markers: Medicinal plant
The genus Alcea that is a member of Malvaceae family, is a Mediterranean perennial herb with major diversity centers in the Middle East and Western Mediterranean Basin. Alcea is a unique genus with several endemic taxa, and many of these endemic taxa from the Irano-Turanian phytogeographical area are particularly interesting. The genus contains 34 species in Iran, 15 of which are endemic. As a result of the importance of Alcea species, molecular data were collected for this genus in this research. For this study, 83 plants were used, which were randomly obtained from 5 species found in 6 provinces. Genomic DNA Amplification with 5 primers resulted in 75 bands, 66 of which were polymorphic (88.12%). The high average MI and PIC values indicated that SCoT primers have a high capacity for detecting polymorphic loci among the species of Alcea. The range of genetic similarities between 5 collected species was estimated to be between 0.77 and 0.89. The SCoT markers analysis revealed that the species Alcea angulata and Alcea popovii had the least similarity, while Alcea loftusii and Alcea sulphurea had the most similarity. The current study?s objectives are as follows: 1) is it possible to identify Alcea species through SCoT markers, 2) what genetic structure do these species have in Iran, and 3) what is the species inter-relationship? The current research found that SCoT markers can be used to identify the species.
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来源期刊
Genetika-Belgrade
Genetika-Belgrade AGRONOMY-GENETICS & HEREDITY
CiteScore
1.80
自引率
0.00%
发文量
1
审稿时长
6-12 weeks
期刊介绍: The GENETIKA is dedicated to genetic studies of all organisms including genetics of microorganisms, plant genetics, animal genetics, human genetics, molecular genetics, genomics, functional genomics, plant and animal breeding, population and evolutionary genetics, mutagenesis and genotoxicology and biotechnology.
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