塞尔维亚核果病原菌Monilinia spp的形态、培养和生态特征

J. Hrustić, M. Mihajlović, B. Tanović
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引用次数: 1

摘要

不同的褐腐病病原体在水果上引起相似的症状,这使得仅根据症状来区分它们很困难。因此,介绍了几种准确鉴定念珠菌属的方法。尽管这些方法是可靠的,但它们的使用存在一些局限性,本研究的目的是找出是否有任何特定的特征可以作为精确鉴定和深入表征Monilinia物种的附加特征。结果表明,PDA培养基上的形态特征和菌丝体生长速度可以作为Monilinia菌种分化的重要指标,因为果实分枝杆菌的生长速度比其他两种菌株快,而果实分枝杆菌的生长速度最慢。麦支霉和果支霉的最佳培养基为MALT,果支霉的最佳培养基为PDA。在极酸性培养基(pH 2)中,只有分离菌株和分离菌株能在其中生长,而在中碱性培养基(pH 9)中,只有分离菌株能在其中生长。结果表明,分离菌株和分离菌株的最佳生长温度为23℃,而分离菌株的最佳生长温度为28℃。另一方面,在极端温度下,Monilinia分离株的敏感性存在一些差异:M. fructigena分离株最敏感(从4?C ~ 31℃),而果分枝杆菌分离株的抗性最强(生长温度为2℃)。C)。所得结果推断,没有特定的特征可以用来可靠和精确地鉴定Monilinia种,但我们观察到极端温度和培养基pH值对不同物种的影响有所不同。需要进一步的研究,包括更多的分离株,才能得出最终结论。
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Morphological, cultural and ecological characterization of Monilinia spp., pathogens of stone fruit in Serbia
Different brown rot pathogens cause similar symptoms on fruit, which makes it difficult to differentiate them based only on developed symptoms. Therefore, several methods have been described for accurate identification of Monilinia species. In spite of the fact that those methods can be reliable, there are several limitations for their use, and the aim of this study was to find out if there are any specific characteristics that could be used as additional features for precise identification and in-depth characterization of Monilinia species. The results showed that morphological characteristics on PDA, and mycelium growth rate on PDA medium can be useful characters for differentiation of Monilinia species since M. fructicola was found to grow faster than the other two test species, while M. fructigena grew the slowest. MALT was the optimal medium for M. laxa and M. fructigena isolates, while PDA medium was optimal for M. fructicola. Regarding an extremely acidic medium (pH 2), only M. laxa and M. fructicola isolates were able to grow in it, while M. fructigena isolate was the only that grew in a moderately alkaline medium (pH 9). Also, the results revealed that the optimal temperature for M. fructigena and M. fructicola growth was 23oC, while 28oC was optimal for M. laxa isolate. On the other hand, some differences in the sensitivity of Monilinia isolates were revealed at extreme temperatures: M. fructigena isolate was the most sensitive (grew from 4?C to up to 31?C), while M. fructicola isolate was the most resistant (grew at 2?C and 34?C). The obtained results inferred that there are no specific features that can be used for reliable and precise identification of Monilinia species, but we observed some differences regarding the effects of extreme temperatures and pH values of culture medium on different species. Further research, involving more isolates, is needed for a final conclusion.
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