双硫磷潜在遗传毒性的评估

Hayal Cobanoglu, A. Çayır
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引用次数: 0

摘要

农药被广泛用于家庭和工业用途,其基因毒性效应引起了公众健康的极大关注。双硫磷是有机磷农药的一种,是最广泛使用的一组化学物质,用于对付农业和家庭昆虫。因此,在本研究中,我们旨在研究双硫磷对人外周血淋巴细胞的遗传毒性和细胞毒性作用,采用细胞分裂阻断微核(CBMN)和姐妹染色单体交换试验。结果表明,在浓度为50和75 μg/ml时,微核(MN)频率增加,但无统计学意义(p < 0.05)。我们发现,姐妹染色单体交换(SCE)值在50和75 μg/ ml浓度下显著高于对照组(p0.05)。结果表明,双硫磷在25、50和75 μg/ml浓度下均无细胞毒性。然而,它可能对人类外周淋巴细胞具有遗传毒性。
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Assessment of the genotoxic potential of temephos
Genotoxic effects of pesticides are of great concern for public health due to the fact that they are widely used for both domestic and industrial purposes. Temephos is a member of organophosphorus pesticides, which is the most widely used group of chemicals against both agricultural and domestic insects. We therefore aimed in the present study to investigate the genotoxic and cytotoxic effects of temephos on human peripheral blood lymphocytes, using the cytokinesis-block micronucleus (CBMN) and sister chromatid exchange assays. The results showed that micronucleus (MN) frequency increased at concentrations of 50 and 75 μg/ml although it was not found statically significant (p>0.05). We found that sister chromatid exchange (SCE) values at concentrations of 50 and 75 μg/ ml were significantly higher than those obtained for the control (p<0.01). We also analyzed associations between temephos exposure and mitotic index (MI), proliferation index (PI), and cell blocked proliferation index (CBPI). There was no significant change in these values at the tested concentrations (p>0.05). It can be concluded that temephos was not cytotoxic at concentrations of 25, 50 and 75 μg/ml. However, it may have a genotoxic potential in human peripheral lymphocytes.
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审稿时长
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