pyrithione铜对卵泡旁虾精子毒性、胚胎毒性和细胞毒性的研究(Lamarck, 1816)

Ezgi Taşcı, S. Hayretdağ
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引用次数: 0

摘要

采用生物测定方法,评价了广泛应用的生物污染杀菌剂铜吡啶硫酮(CuPt)的精子毒、胚胎毒和细胞毒作用,考察了其对海胆受精率、后代质量和早期发育的抑制作用。CuPt对受精率没有精子毒性,但当卵子与暴露于CuPt的精子受精时,胚胎畸形的频率以浓度依赖性的方式增加。胚胎毒性CuPt EC50为13.58 g/l。正常发育的赘肉出现的频率降低,骨骼变形的幼虫数量增加。细胞毒性试验的IC25和IC50分别为12.79和47.85 g/l。研究显示,暴露的细胞中有丝分裂细胞数量显著减少,间期细胞百分比增加,染色体异常增加。根据这些结果,CuPt可以说在施用浓度下对海胆胚胎具有高度毒性作用。这一情况表明,该物种可能存在CuPt污染海洋的潜在风险。
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Investigation of spermiotoxic, embryotoxic and cytotoxic effects of copper pyrithione on Paracentrotus lividus (Lamarck, 1816)
Spermiotoxic, embryotoxic and cytotoxic effects of the widely used biofouling biocide copper pyrithione (CuPt) were evaluated in bioassays to examine the inhibition of fertilization rate, offspring quality and effects on early development of the sea urchin Paracentrotus lividus. CuPt was non-spermiotoxic for fertilization rates but the frequency of embryonic malformations increased in a concentration-dependent manner when eggs were fertilized with CuPt-exposed sperm. CuPt EC50 was calculated to be 13.58 ?g/l for embryotoxicity. While the frequency of normally developed plutei decreased, the number of larvae with skeletal deformations increased. The IC25 and IC50 values in cytotoxicity assays were calculated to be 12.79 and 47.85 ?g/l, respectively. The study revealed statistically significant decrease in the number of mitotically dividing cells, increase in the percentage of interphase cells and increased chromosomal abnormalities in the exposed cells. According to these results, CuPt can be said to have a highly toxic effect on sea urchin embryos at the applied concentrations. This situation suggests that there may be a potential risk of marine contamination with CuPt for this species.
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