天然山楂紫丁香克隆结构及遗传多样性的ISSR分析

Yajun Yang, Ji-Ning Li, L. Gong, Jian Zhou
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引用次数: 1

摘要

利用ISSR分子标记对8个天然山雀花居群的克隆多样性、生长结构和遗传多样性进行了评价。结果表明,3个基因的16个分株具有相同的基因型,7个居群的239个样品中有136个基因型分化。克隆多样性表明,山楂属方阵无性系,每个居群由多个无性系组成。多数基因含有1个分型,单个基因最多含有8个分型。基因型Simpson指数克隆多样性较高(D=0.994),基因型Fager指数分布均匀性较高(E= 0.985)。在种水平上,每基因型个体数(NC)和平均基因型比例(PD)分别为1.729和0.578。此外,在种群和物种水平上均存在较高的遗传多样性。基因分化系数(GST)为0.320,表明群体间分化小于群体内分化。
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Clonal Structure and Genetic Diversity of Natural Syringa pinnatifolia var. alanshanica Assessed by ISSR
Clonal diversity,growth structure,and genetic diversity of 8 natural Syringa pinnatifolia var.alanshanica populations were assessed by ISSR molecular markers.Results showed that 16 ramets of 3 individual genets shared the same genotype,whereas 136 genotypes were differentiated among the other 239 samples from 7 populations.Clonal diversity revealed that S.pinnatifolia var.alanshanica was a phalanx clonal species and each population was composed of several clones.Most genets contained one ramet and the highest quantity of one genet consisted of 8 ramets.A relatively high clonal diversity of genotype Simpson's index(D=0.994) and distributional uniformity of genotype Fager's index(E= 0.985) was detected.Individuals per genotype(NC) and mean genotype proportion(PD) were 1.729 and 0.578,respectively,at the species level.In addition,high genetic diversity was detected at both population and species level.The coefficient gene differentiation(GST) was 0.320,indicating less differentiation among populations than within populations.
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