Composition of Bacterial Community in Musca domestica Midgut

Housefly(Musca domestica) is an important mechanical and biological vector harboring and transmitting human and animal pathogens. To study the composition of bacterial community in housefly midgut and assess the role of housefly in the epidemiology of diseases, 16S rRNA gene clone library of bacteria in housefly midgut was constructed. The library compromised of 88 sequences, which were grouped into 22 OTUs, including Proteobacteria(12 OTUs), Firmicutes(9 OTUs), and Bacteroidetes(1 OTU). Twelve OTUs of Proteobacteria belonged to Betaproteobacteria(1 OTU), Epsinoproteobacteria(1 OTU)and Gammaproteobacteria(10 OTUs) respectively. No Alphaproteobacteria or Deltaproteobacteria was found. The 9 Firmicutes included Lactobacillales(6 OTUs), Clostridia(1 OTU) and Bacillales(2 OTUs). In the library, Gammaproteobacteria was the dominant phylotype, which compromised 71 sequences(80.7%). Some species in Enterobacter, Klebsiella, Providencia, Proteus,Aeromonas, and Staphylococcus in the library were pathogens or opportunistic pathogens to human and animal. These results help to understand the significant roles of housefly in disease transmitting.