两种商业免疫测定法定量人唾液中类固醇激素的实际评价

Q3 Biochemistry, Genetics and Molecular Biology Journal of Cellular Biotechnology Pub Date : 2021-01-01 DOI:10.3233/jcb-209012
Jonas Schmidt, F. Wenzel, F. Blessing
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引用次数: 0

摘要

背景:在临床诊断中使用唾液作为样本进行类固醇激素分析存在一定的问题。因此,在常规实验室中测试当前可用的分析方法是否适用于这种方法是很重要的。目的:比较两种市售免疫测定法在常规条件下的分析性能。此外,将唾液类固醇激素水平与血清水平进行比较。方法:采集健康受试者的唾液和血清。随后,使用ADIVA Centaur®XP平台和市售手动ELISA试剂盒检测唾液中皮质醇、睾酮、孕酮和雌二醇的浓度。使用ADVIA Centaur®平台测定所选类固醇激素的血清水平。结果:目前,由于校准范围不足,ADVIA Centaur®XP平台不能用于唾液中类固醇激素的定量。另一方面,市售的ELISA试剂盒非常适合这种应用。结论:目前市售的免疫检测方法具有足够的灵敏度,可用于唾液中类固醇激素的检测,且可在常规条件下使用。然而,由于结果的巨大可变性,唾液类固醇激素水平的解释仍然是一项具有挑战性的任务。
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Practical evaluation of two commercial immunoassays for the quantification of steroid hormones in human saliva
BACKGROUND: There are certain issues using saliva as a sample for steroid hormone analysis in clinical diagnostics. Therefore, it is important to test whether currently available assays are suitable for this approach in a routine laboratory. OBJECTIVES: The analytical performance of two commercially available immunoassays was compared under routine conditions. In addition, salivary steroid hormone levels were compared with serum levels. METHODS: Saliva and serum samples were collected from healthy subjects. Afterwards, the concentration of cortisol, testosterone, progesterone and estradiol in saliva was measured by using the ADIVA Centaur® XP platform and commercially available manual ELISA kits. Serum levels of the selected steroid hormones were determined by using the ADVIA Centaur® platform. RESULTS: Currently, the ADVIA Centaur® XP platform cannot be used for the quantification of steroid hormones in saliva due to an insufficient calibration range. Commercially available ELISA kits on the other side are well suitable for this application. CONCLUSIONS: There are commercially available immunoassays which are sensitive enough for the determination of steroid hormones from saliva and can be used under routine conditions. However, the interpretation of salivary steroid hormone levels stays a challenging task due to the large variability of the results.
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来源期刊
Journal of Cellular Biotechnology
Journal of Cellular Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
0.70
自引率
0.00%
发文量
13
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