EFFECT OF ALA-GLN FEEDING ON GROWTH, ANTIOXIDANT LEVEL,AND IMMUNITY OF CYPRINUS CARPIO VAR. JIAN

This study was carried out using healthy Cyprinus with the initial body weight of(33.52±0.17) g in a single recirculating system for 8 weeks.Diets with equal levels of protein(35 g/kg) and energy(17 kJ/g) were administered to the Cyprinus at concentrations of 0.0 %(control diet) and 0.5%(trial diet) Ala-Gln of dietary dry matter in five different feeding modes: continuous 8 weeks of control diet(group I or control),trial diet at an interval of 2 weeks(group II),trial diet at 4 weeks and then control diet at 4 weeks(group III),the contrary to group Ⅲ(group IV) and continuous 8-week trial diet(group V).The trail was conducted to investigate the effects of different feeding mode of Ala-Gln on growth,immunity and antioxidant in Cyprinus.Our results indicated that growth rates under all different feeding modes of Ala-Gln were significantly higher than that of the control group(P0.05).Growth rate of Ⅱ was higher than that of Ⅲ,Ⅳ,Ⅴgroups(P0.05),and growth rate of Ⅲ was higher than that of Ⅴgroup(P0.05).Serum SOD of the Ⅳ and Ⅴgroups were significantly higher than the control group(P0.05),and serum SOD of the Ⅴgroup was significantly higher than those of Ⅱ and Ⅲ groups(P0.05).GSH-Px in hepatopancreas of different feeding modes were significantly higher than that of the control group(P0.05),Serum GSH-Px of Ⅳ and Ⅴgroups were higher than that of the control group(P0.05).LZM in the head kidneys of the Ala-Gln groups were higher than the control group,only group Ⅱ showed no significant difference(P0.05),LZM in the spleen of the groups Ⅳ and Ⅴ were significantly higher than that of the control group(P0.05).In the current experiment,we concluded that adding Ala-Gln could improve the growth,immunity and antioxidant levels of Cyprinus.There were significant differences of different feeding modes.Given economical efficiency and practicability,feeding mode with 2-week intervals were suggested in the practical aquaculture of Cyprinus.