中国恒河猴静脉接种SHIV1157ipd3N4原液的测定

中国比较医学杂志 Pub Date : 2011-01-01 DOI:10.3969/J.ISSN.1671.7856.2011.04.003

Z. Cong, H. Jiang, G. Jin, T. Chen, W. Wang, Z. Tao, Nailin Yao, J. Xiong, F. Wu, Zhiwei Chen, Q. Wei

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引用次数: 1

摘要

目的测定SHIV1157ipd3N4静脉注射感染中国恒河猴的病毒浓度，阐明SHIV1157ipd3N4接种后猴体内的病毒复制和免疫功能受损情况。方法以10只中国猕猴为研究对象。首先，对所有猴子进行血清学检测，确保它们不携带SIV、SRV和STLV。然后分别用10倍系列稀释的SHIV1157ipd3N4感染1 mL。最后进行血常规检查，并采用实时RT-PCR检测病毒载量。结果5 TCID50 /mL以上的SHIV1157ipd3N4可成功体外感染中国恒河猴。结论本研究结果为建立中国恒河猴静脉感染SHIV1157ipd3N4模型提供了坚实的基础。该模型对HIV-1亚型C疫苗及其发病机制的研究具有重要意义。

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Titration of a SHIV1157ipd3N4 Stock by Intravenous Inoculation of Chinese-Origin Rhesus Macaques

Objective To determine the viral concentration of SHIV1157ipd3N4 to infect Chinese-origin rhesus macaques intravenously,and to clarify the viral replication and immunological impairment in the monkeys after SHIV1157ipd3N4 inoculation.Methods 10 Chinese-origin rhesus macaques were used in this study.Firstly,all the monkeys were screened by serological test to ensure that they were free of SIV,SRV,and STLV.Then they were infected with 1 mL of 10-fold serial dilution of SHIV1157ipd3N4 separately.Finally to make blood routine examination and measure the viral loads of the monkeys by real-time RT-PCR.Results More than 5 TCID50 /mL of SHIV1157ipd3N4 can infect the Chinese-origin rhesus macaques i.v.successfully.Conclusions Our findings of this study provide a firm basis for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus macaques intravenously.This model would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.

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