{"title":"利用基于pcr的ITS和RAPD技术分析16个非洲花木种源的地理变异","authors":"O. Dangasuk, P. Odago, B. Were, S. Gudu","doi":"10.4314/DAI.V19I2.15791","DOIUrl":null,"url":null,"abstract":"PCR analysis based on ITS and RAPD were carried out on sixteen provenances of Faidherbia albida currently growing in a provenance trial in semi-arid Baringo district, Kenya. The objectives of the study were: 1. Determination of the phylogenetic relationship among the 16 provenances in order to establish the species centre of origin 2. Determination of extent of genetic diversity in F. albida using PCR markers. ITS data did not produce any consistent regional or geographic pattern. RAPD data produced a dendrogram clearly grouping the provenances into Western, Southern and Eastern African regions.\nThe study revealed the utility of RAPD markers in understanding geographic variation and phylogenetic relationships among Faidherbia albida populations in Africa. Keywords : DNA, Faidherbia albida , genetic diversity, ITS, PCR, provenance, RAPD Discovery and Innovation Vol. 19 (2) 2007: pp. 108-116","PeriodicalId":50577,"journal":{"name":"Discovery and Innovation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Analysis of Geographic Variation among Sixteen African Provenances of Faidherbia albida using PCRBased ITS And RAPD Techniques\",\"authors\":\"O. Dangasuk, P. Odago, B. Were, S. Gudu\",\"doi\":\"10.4314/DAI.V19I2.15791\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"PCR analysis based on ITS and RAPD were carried out on sixteen provenances of Faidherbia albida currently growing in a provenance trial in semi-arid Baringo district, Kenya. The objectives of the study were: 1. Determination of the phylogenetic relationship among the 16 provenances in order to establish the species centre of origin 2. Determination of extent of genetic diversity in F. albida using PCR markers. ITS data did not produce any consistent regional or geographic pattern. RAPD data produced a dendrogram clearly grouping the provenances into Western, Southern and Eastern African regions.\\nThe study revealed the utility of RAPD markers in understanding geographic variation and phylogenetic relationships among Faidherbia albida populations in Africa. Keywords : DNA, Faidherbia albida , genetic diversity, ITS, PCR, provenance, RAPD Discovery and Innovation Vol. 19 (2) 2007: pp. 108-116\",\"PeriodicalId\":50577,\"journal\":{\"name\":\"Discovery and Innovation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-02-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Discovery and Innovation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4314/DAI.V19I2.15791\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Discovery and Innovation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/DAI.V19I2.15791","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
摘要
利用ITS和RAPD对肯尼亚半干旱Baringo地区一个种源试验中生长的16个花木进行了PCR分析。本研究的目的是:1。确定16个种源之间的系统发育关系，以建立物种起源中心。用PCR标记测定花楸遗传多样性程度。ITS数据没有产生任何一致的区域或地理格局。RAPD数据产生的树形图清楚地将种源划分为西部、南部和东部非洲地区。本研究揭示了RAPD标记在了解非洲白翅蝗种群的地理变异和系统发育关系中的应用。关键词:DNA，白翅草，遗传多样性，ITS, PCR，种源，RAPD，发现与创新Vol. 19 (2) 2007: pp. 108-116
Analysis of Geographic Variation among Sixteen African Provenances of Faidherbia albida using PCRBased ITS And RAPD Techniques
PCR analysis based on ITS and RAPD were carried out on sixteen provenances of Faidherbia albida currently growing in a provenance trial in semi-arid Baringo district, Kenya. The objectives of the study were: 1. Determination of the phylogenetic relationship among the 16 provenances in order to establish the species centre of origin 2. Determination of extent of genetic diversity in F. albida using PCR markers. ITS data did not produce any consistent regional or geographic pattern. RAPD data produced a dendrogram clearly grouping the provenances into Western, Southern and Eastern African regions.
The study revealed the utility of RAPD markers in understanding geographic variation and phylogenetic relationships among Faidherbia albida populations in Africa. Keywords : DNA, Faidherbia albida , genetic diversity, ITS, PCR, provenance, RAPD Discovery and Innovation Vol. 19 (2) 2007: pp. 108-116
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