Cytological effects of the root extracts of Boerhaavia diffusa on root tips of Crinum jagus

The mitotic effects of the root extracts of Boerhaavia diffusa on the root tips of Crinum jagus were investigated. The results of this study showed several chromosomal abnormalities including stickiness of chromosomes (both at metaphase and anaphase), C-metaphase, lagging chromosomes, and sticky bridges. The trend of the results showed that the higher the concentration of the extracts for treatment, the more inhibitory the effect on mitosis with more pronounced chromosomal aberrations. The root extract of B. diffusa was found active at concentrations of 0.1%, 0.2%, 0.4%, and 0.8%. The extract was found to accumulate metaphase and could thus be of immense help in cytological work. These results are discussed in the light of their reported use in cancer therapy, the treatment of other diseases in traditional medicinal practice and its' possible use as an alternative to colchicine in cytological work.