枯草芽孢杆菌RSKK96固态发酵产胞外碱性蛋白酶的研究

Q3 Agricultural and Biological Sciences Eurasian Journal of Biosciences Pub Date : 2011-07-01 DOI:10.5053/EJOBIOS.2011.5.0.8
N. Akcan, F. Uyar
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引用次数: 56

摘要

采用固态发酵法对枯草芽孢杆菌RSKK96产胞外碱性蛋白酶进行了研究。研究了不同的农业残基作为底物生产酶。以小扁豆壳为单位每质量干底物产酶量最高(3937.0 U/mg)。优化生产参数为:培养时间120 h,提取介质Triton-X100为1%,初始含水率30%,初始pH为9.0。在含有阿拉伯糖、乳糖、半乳糖和果糖的培养基中获得高水平的碱性蛋白酶。在不同的氮源中,牛肉提取物是碱性蛋白酶的最佳诱导剂,而其他氮源对酶的产生有抑制作用。在金属盐中,FeSO4.7H2O和MgSO4.7H2O可以提高蛋白酶的产量。在1000 mL发酵培养基体积下,扁豆壳的产酶量最高,为5759.2 U/mg。
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Production of extracellular alkaline protease from Bacillus subtilis RSKK96 with solid state fermentation
The production of extracellular alkaline protease by producing Bacillus subtilis RSKK96 was studied with solid state fermentation (SSF). Different agro residues as substrate were studied for enzyme production. The highest enzyme production was expressed with lentil husk as units per mass of dry substrate (3937.0 U/mg). Production parameters were optimized as incubation time 120 h, extraction medium Triton-X100 1%, initial moisture content 30%, initial pH 9.0. The high level of alkaline protease was obtained in the medium containing arabinose followed by lactose, galactose, and fructose. Among various nitrogen sources, beef extract was found to be the best inducer of alkaline protease, while other nitrogen sources repressed enzyme production. Among metal salts FeSO4.7H2O and MgSO4.7H2O was found to increase protease production. The maximum enzyme production (5759.2 U/mg) was observed with lentil husk in 1000 mL of fermentation medium volume.
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
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0
期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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