Effect of combination of Indigofera zollingeriana, black soldier fly larvae, and turmeric on performance and histomorphological characterizes of native chicken at starter phase

Sources of high protein feed ingredients can come from plants and animals (insects), namely Indigofera zollingeriana syn. and black soldier fly larvae. The addition of natural feed additives to feed can be obtained from turmeric phytobiotics which have many biological activities, such as anticancer, anti-inflammatory, antimicrobial and antioxidant. This study aimed to determine the effect of the combination of Indigofera zollingeriana syn., black soldier fly (BSF) larvae and turmeric on the performance and histomorphology of the bursa Fabricius in the native chicken starter phase. The research design was completely randomized with 3 treatments and 5 replications with 6 chickens per unit. The combination treatments were P0 (Commercial feed as control); P1 (5% Indigofera flour + 25% BSF larvae flour + 2.5% turmeric flour) and P2 (10% Indigofera flour + 20% BSF larvae flour + 2.5% turmeric flour). Parameters measured in this study were performance (body weight gain, feed consumption, FCR) and bursa of fabricius histomorphology in native chickens. The results of the analysis of variance showed that the combination of Indigofera zollingeriana syn., BSF larvae and turmeric had a significant effect on the performance of native chickens but could not match the performance of P0 (control feed). While the histomorphology of bursa Fabricius showed that the combination of Indigofera zolliengeriana flour up to 10% and 25% black soldier fly larvae flour in the feed identified the medulla width, cortex thickness and follicle width can increase lymphocyte cells to produce antibodies for native chickens at starter phase.