Study the Effect of Zwitterionic Hydrophilic Interaction for Simultaneous Determination of Non-Steroidal Anti-Inflammatory Drugs in Dosage Forms

Flurbiprofen and ketorolac are non-steroidal anti-inflammatory drugs (NSAIDs) that include both hydrophilic and hydrophobic functional groups in their structures. By connecting sulfobetaine molecules to polystyrene-divinylbenzene particles, two ZIC-HILIC stationary phases (ZIC-SB1 and ZIC-SB4) were developed for chromatographic separation of NSAIDs with varying chain lengths using Ultraviolet as detection. The different chain lengths of the stationary phases are utilized to investigate the retention behaviour of NSAIDs. Simultaneous quantification methods for ZIC-HILIC were developed. Flurbiprofen and ketorolac exhibited high precision with a small relative standard deviation (RSD) value of 0.48%, linear ranges of 0.015-6 and 0.06-4.5 ppm and detection limits of 0.009-0.006 and 0.01-0.008 ppm with a coefficient of determination (R2) of 0.9995-0.9997 for the ZIC-SB1 and ZIC-SB4 stationary phases, respectively. Statistical testing was used to compare the approaches to the British Pharmacopoeia protocol, and no difference in accuracy was discovered.