{"title":"首次从印度东北部 COVID-19 患者体内分离出 SARS-CoV-2 并确定其特征。","authors":"Aniruddha Jakharia, Biswajyoti Borkakoty, Sumi Pait, Gautam Baruah, Rahul Hazarika, Dipankar Biswas","doi":"10.4103/ijpm.ijpm_748_21","DOIUrl":null,"url":null,"abstract":"<p><p>Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) responsible for the current pandemic has resulted in over 5 million deaths globally. More than a year has passed, still SARS-CoV-2 panic the public life. Virus isolation is of paramount importance for development of vaccines, in-vitro screening of antiviral compounds, pathogenesis studies, etc., Many cell lines were studied for amplification and replication of SARS-CoV-2 and Vero cells were found to be ideal cell lines for isolation. In May 2020, ICMR-Regional Medical Research Centre, NE region, India, successfully established the SARS-CoV-2 culture system in Vero CCL-81 cell lines. Phylogenetic analyses of the whole genome sequences of the SARS-CoV-2 isolate (EPI_ISL_2501532 | 2020-05-19) showed monophyletic clade G and lineage B.1.1.</p>","PeriodicalId":13488,"journal":{"name":"Indian Journal of Pathology and Microbiology","volume":"1 1","pages":"133-139"},"PeriodicalIF":0.8000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"First isolation and characterization of SARS-CoV-2 from COVID-19 patient of North East India.\",\"authors\":\"Aniruddha Jakharia, Biswajyoti Borkakoty, Sumi Pait, Gautam Baruah, Rahul Hazarika, Dipankar Biswas\",\"doi\":\"10.4103/ijpm.ijpm_748_21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) responsible for the current pandemic has resulted in over 5 million deaths globally. More than a year has passed, still SARS-CoV-2 panic the public life. Virus isolation is of paramount importance for development of vaccines, in-vitro screening of antiviral compounds, pathogenesis studies, etc., Many cell lines were studied for amplification and replication of SARS-CoV-2 and Vero cells were found to be ideal cell lines for isolation. In May 2020, ICMR-Regional Medical Research Centre, NE region, India, successfully established the SARS-CoV-2 culture system in Vero CCL-81 cell lines. Phylogenetic analyses of the whole genome sequences of the SARS-CoV-2 isolate (EPI_ISL_2501532 | 2020-05-19) showed monophyletic clade G and lineage B.1.1.</p>\",\"PeriodicalId\":13488,\"journal\":{\"name\":\"Indian Journal of Pathology and Microbiology\",\"volume\":\"1 1\",\"pages\":\"133-139\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Pathology and Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.4103/ijpm.ijpm_748_21\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PATHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Pathology and Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4103/ijpm.ijpm_748_21","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PATHOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
严重急性呼吸系统综合症冠状病毒-2（SARS-CoV-2）是造成当前大流行病的罪魁祸首，已导致全球 500 多万人死亡。一年多过去了，SARS-CoV-2 依然肆虐公众生活。病毒分离对于疫苗开发、抗病毒化合物体外筛选、发病机理研究等至关重要，许多细胞系都对 SARS-CoV-2 的扩增和复制进行了研究，发现 Vero 细胞是分离病毒的理想细胞系。2020 年 5 月，印度东北部地区的 ICMR 区域医学研究中心成功地在 Vero CCL-81 细胞系中建立了 SARS-CoV-2 培养系统。对 SARS-CoV-2 分离物（EPI_ISL_2501532 | 2020-05-19）的全基因组序列进行的系统发生学分析表明，该分离物属于单系 G 支系和 B.1.1 系。
First isolation and characterization of SARS-CoV-2 from COVID-19 patient of North East India.
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) responsible for the current pandemic has resulted in over 5 million deaths globally. More than a year has passed, still SARS-CoV-2 panic the public life. Virus isolation is of paramount importance for development of vaccines, in-vitro screening of antiviral compounds, pathogenesis studies, etc., Many cell lines were studied for amplification and replication of SARS-CoV-2 and Vero cells were found to be ideal cell lines for isolation. In May 2020, ICMR-Regional Medical Research Centre, NE region, India, successfully established the SARS-CoV-2 culture system in Vero CCL-81 cell lines. Phylogenetic analyses of the whole genome sequences of the SARS-CoV-2 isolate (EPI_ISL_2501532 | 2020-05-19) showed monophyletic clade G and lineage B.1.1.
期刊介绍：
The journal will cover studies related to pathology including morbid anatomy, surgical pathology, clinical pathology, diagnostic cytopathology including gynecologic cytology and aspiration cytology, hematology including immuno-hematology and medical microbiology. The journal gives preference to clinically oriented studies over experimental and animal studies. The Journal would publish peer-reviewed original research papers, case reports, systematic reviews, meta-analysis, letters to the editor and brief communications. Review articles on current topics usually are invited by the editor.
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