脊索瘤细胞中参考基因的鉴定有助于对不同亚型的表达研究进行交叉比较。

IF 0.9 4区医学 Q4 CLINICAL NEUROLOGY Turkish neurosurgery Pub Date : 2024-01-01 DOI:10.5137/1019-5149.JTN.42049-22.3

Didem Seven, Nehir Kizililsoley, Emrah Nikerel, Omer Faruk Bayrak, Ugur Ture

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引用次数: 0

摘要

材料与方法：我们调查了公共脊索瘤阵列转录组数据集中的13个候选参考基因，通过RT-PCR对这些基因进行了验证，并使用NormFinder、geNorm和Bestkeeper对其稳定性进行了评估：我们调查了公共脊索瘤阵列转录组数据集中的13个候选参考基因，通过RT-PCR验证了这些基因，并用NormFinder、geNorm和Bestkeeper评估了它们的稳定性：结果：通过三种不同的方法，YWHAZ、TBP和PGK1基因被确认为最稳定的参考基因。相反，KRT8、KRT19 和 GAPDH 基因稳定性较差，不适合用于脊索瘤研究：结论：对于脊索瘤基因图谱分析中的 RT-PCR 实验归一化，我们建议使用稳定的基因 YWHAZ、TBP 和 PGK1。

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Identification of Reference Genes in Chordoma Cells Allows Cross-Comparison of Expression Studies Across Subtypes.

Aim: To present the best housekeeping genes including clival/sacral based chordoma, and the nucleus pulposus cells.

Material and methods: We investigated 13 candidate reference genes in public chordoma array transcriptome datasets, validated these genes by using RT-PCR, and evaluated their stability with NormFinder, geNorm, and Bestkeeper.

Results: YWHAZ, TBP and PGK1 genes were identified as the most stable reference genes as confirmed with three different approaches. Conversely, KRT8, KRT19 and GAPDH genes are less stable and not appropriate for use in chordoma research.

Conclusion: For normalization of RT-PCR experiments in gene profiling of chordoma, we recommend the use of the stable genes YWHAZ, TBP and PGK1.

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来源期刊

Turkish neurosurgery 医学-临床神经学

CiteScore

1.50

自引率

12.50%

发文量

126

审稿时长

2 months

期刊介绍： Turkish Neurosurgery is a peer-reviewed, multidisciplinary, open access and totally free journal directed at an audience of neurosurgery physicians and scientists. The official language of the journal is English. The journal publishes original articles in the form of clinical and basic research. Turkish Neurosurgery will only publish studies that have institutional review board (IRB) approval and have strictly observed an acceptable follow-up period. With the exception of reference presentation, Turkish Neurosurgery requires that all manuscripts be prepared in accordance with the Uniform Requirements for Manuscripts Submitted to Biomedical Journals.